Aufsatz
Candidates for photic entrainment pathways to the circadian clock via optic lobe neuropils in the Madeira cockroach
Zusammenfassung
The compound eye of cockroaches is obligatory for entrainment of the Madeira cockroach's circadian clock, but the cellular nature of its entrainment pathways is enigmatic. Employing multiple‐label immunocytochemistry, histochemistry, and backfills, we searched for photic entrainment pathways to the accessory medulla (AME), the circadian clock of the Madeira cockroach. We wanted to know whether photoreceptor terminals could directly contact pigment‐dispersing factor‐immunoreactive (PDF‐ir) circadian pacemaker neurons with somata in the lamina (PDFLAs) or somata next to the AME (PDFMEs). Short green‐sensitive photoreceptor neurons of the compound eye terminated in lamina layers LA1 and LA2, adjacent to PDFLAs and PDFMEs that branched in LA3. Long UV‐sensitive compound eye photoreceptor neurons terminated in medulla layer ME2 without direct contact to ipsilateral PDFMEs that arborized in ME4. Multiple neuropeptide‐ir interneurons branched in ME4, connecting the AME to ME2. Before, extraocular photoreceptors of the lamina organ were suggested to send terminals to accessory laminae. There, they overlapped with PDFLAs that mostly colocalized PDF, FMRFamide, and 5‐HT immunoreactivities, and with terminals of ipsi‐ and contralateral PDFMEs. We hypothesize that during the day cholinergic activation of the largest PDFME via lamina organ photoreceptors maintains PDF release orchestrating phases of sleep–wake cycles. As ipsilateral PDFMEs express excitatory and contralateral PDFMEs inhibitory PDF autoreceptors, diurnal PDF release keeps both PDF‐dependent clock circuits in antiphase. Future experiments will test whether ipsilateral PDFMEs are sleep‐promoting morning cells, while contralateral PDFMEs are activity‐promoting evening cells, maintaining stable antiphase via the largest PDFME entrained by extraocular photoreceptors of the lamina organ.
Zitierform
In: JCN The journal of comparative neurology Volume 528 / Issue 10 (2019-12-20) , S. 1754-1774 ; EISSN 1096-9861Förderhinweis
Gefördert im Rahmen des Projekts DEALZitieren
@article{doi:10.17170/kobra-202005081235,
author={Arnold, Thordis and Korek, Sebastian and Massah, Azar and Eschstruth, David and Stengl, Monika},
title={Candidates for photic entrainment pathways to the circadian clock via optic lobe neuropils in the Madeira cockroach},
journal={JCN The journal of comparative neurology},
year={2019}
}
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2020-05-20T06:26:10Z 2020-05-20T06:26:10Z 2019-12-20 doi:10.17170/kobra-202005081235 http://hdl.handle.net/123456789/11563 Gefördert im Rahmen des Projekts DEAL eng Namensnennung 4.0 International http://creativecommons.org/licenses/by/4.0/ 5-HT acetylcholinesterase histochemistry circadian entrainment pathways GABA neuropeptides optic lobe neuropils PDF RRID:AB_177540 RRID:AB_2313973 RRID:AB_2314414 RRID:AB_2314803 RRID:AB_2315017 RRID:AB_2532101 RRID:AB_261181 RRID:AB_477522 RRID:AB_477652 RRID:AB_528479 RRID:AB_760350 570 Candidates for photic entrainment pathways to the circadian clock via optic lobe neuropils in the Madeira cockroach Aufsatz The compound eye of cockroaches is obligatory for entrainment of the Madeira cockroach's circadian clock, but the cellular nature of its entrainment pathways is enigmatic. Employing multiple‐label immunocytochemistry, histochemistry, and backfills, we searched for photic entrainment pathways to the accessory medulla (AME), the circadian clock of the Madeira cockroach. We wanted to know whether photoreceptor terminals could directly contact pigment‐dispersing factor‐immunoreactive (PDF‐ir) circadian pacemaker neurons with somata in the lamina (PDFLAs) or somata next to the AME (PDFMEs). Short green‐sensitive photoreceptor neurons of the compound eye terminated in lamina layers LA1 and LA2, adjacent to PDFLAs and PDFMEs that branched in LA3. Long UV‐sensitive compound eye photoreceptor neurons terminated in medulla layer ME2 without direct contact to ipsilateral PDFMEs that arborized in ME4. Multiple neuropeptide‐ir interneurons branched in ME4, connecting the AME to ME2. Before, extraocular photoreceptors of the lamina organ were suggested to send terminals to accessory laminae. There, they overlapped with PDFLAs that mostly colocalized PDF, FMRFamide, and 5‐HT immunoreactivities, and with terminals of ipsi‐ and contralateral PDFMEs. We hypothesize that during the day cholinergic activation of the largest PDFME via lamina organ photoreceptors maintains PDF release orchestrating phases of sleep–wake cycles. As ipsilateral PDFMEs express excitatory and contralateral PDFMEs inhibitory PDF autoreceptors, diurnal PDF release keeps both PDF‐dependent clock circuits in antiphase. Future experiments will test whether ipsilateral PDFMEs are sleep‐promoting morning cells, while contralateral PDFMEs are activity‐promoting evening cells, maintaining stable antiphase via the largest PDFME entrained by extraocular photoreceptors of the lamina organ. open access Arnold, Thordis Korek, Sebastian Massah, Azar Eschstruth, David Stengl, Monika Deutsche Forschungsgemeinschaft. Grant Numbers: STE 531/18‐3, STE 531/21‐1, STE 531/25‐1, STE 531/26‐1 Küchenschabe Tagesrhythmus Nervensystem publishedVersion EISSN 1096-9861 Issue 10 JCN The journal of comparative neurology 1754-1774 Volume 528 false
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