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Impact of Pus1 Pseudouridine Synthase on Specific Decoding Events in Saccharomyces cerevisiae

dc.date.accessioned2020-06-29T15:32:58Z
dc.date.available2020-06-29T15:32:58Z
dc.date.issued2020-05-07
dc.identifierdoi:10.17170/kobra-202006291376
dc.identifier.urihttp://hdl.handle.net/123456789/11611
dc.description.sponsorshipGefördert durch den Publikationsfonds der Universität Kasselger
dc.language.isoengeng
dc.rightsNamensnennung 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjecttRNA modificationeng
dc.subjectPUS1eng
dc.subjectpseudouridineeng
dc.subjecttranslationeng
dc.subjectnon-sense suppressioneng
dc.subjectsup70-65eng
dc.subjectmisreadingeng
dc.subjectrapid tRNA decayeng
dc.subject.ddc570
dc.titleImpact of Pus1 Pseudouridine Synthase on Specific Decoding Events in Saccharomyces cerevisiaeeng
dc.typeAufsatz
dcterms.abstractPus1-dependent pseudouridylation occurs in many tRNAs and at multiple positions, yet the functional impact of this modification is incompletely understood. We analyzed the consequences of PUS1 deletion on the essential decoding of CAG (Gln) codons by tRNAGlnCUG in yeast. Synthetic lethality was observed upon combining the modification defect with destabilized variants of tRNAGlnCUG, pointing to a severe CAG-decoding defect of the hypomodified tRNA. In addition, we demonstrated that misreading of UAG stop codons by a tRNAGlnCUG variant is positively affected by Pus1. Genetic approaches further indicated that mildly elevated temperature decreases the decoding efficiency of CAG and UAG via destabilized tRNAGlnCAG variants. We also determined the misreading of CGC (Arg) codons by tRNAHisGUG, where the CGC decoder tRNAArgICG contains Pus1-dependent pseudouridine, but not the mistranslating tRNAHis. We found that the absence of Pus1 increased CGC misreading by tRNAHis, demonstrating a positive role of the modification in the competition against non-synonymous near-cognate tRNA. Part of the in vivo decoding defects and phenotypes in pus1 mutants and strains carrying destabilized tRNAGlnCAG were suppressible by additional deletion of the rapid tRNA decay (RTD)-relevant MET22, suggesting the involvement of RTD-mediated tRNA destabilization.eng
dcterms.accessRightsopen access
dcterms.creatorKhonsari, Bahar
dcterms.creatorKlassen, Roland
dc.relation.doidoi:10.3390/biom10050729
dc.subject.swdTransfer-RNSger
dc.subject.swdPseudouridinger
dc.type.versionpublishedVersion
dcterms.source.identifierEISSN 2218-273X
dcterms.source.issueIssue 5
dcterms.source.journalBiomoleculeseng
dcterms.source.pageinfo729
dcterms.source.volumeVolume 10
kup.iskupfalse


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