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Neither per, nor tim1, nor cry2 alone are essential components of the molecular circadian clockwork in the Madeira cockroach

dc.date.accessioned2020-09-14T14:10:06Z
dc.date.available2020-09-14T14:10:06Z
dc.date.issued2020-08-04
dc.identifierdoi:10.17170/kobra-202009111789
dc.identifier.urihttp://hdl.handle.net/123456789/11813
dc.description.sponsorshipGefördert durch den Publikationsfonds der Universität Kasselger
dc.language.isoengeng
dc.rightsNamensnennung 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectcircadian oscillatorseng
dc.subjectcockroacheseng
dc.subjectcircadian rhythmseng
dc.subjectbiological locomotioneng
dc.subjectgenetic oscillatorseng
dc.subjectgene expressioneng
dc.subjectdouble stranded RNAeng
dc.subjectRNA interferenceeng
dc.subject.ddc570
dc.subject.ddc590
dc.titleNeither per, nor tim1, nor cry2 alone are essential components of the molecular circadian clockwork in the Madeira cockroacheng
dc.typeAufsatz
dcterms.abstractCircadian clocks control rhythms in physiology and behavior entrained to 24 h light–dark cycles. Despite of conserved general schemes, molecular circadian clockworks differ between insect species. With RNA interference (RNAi) we examined an ancient circadian clockwork in a basic insect, the hemimetabolous Madeira cockroach Rhyparobia maderae. With injections of double-stranded RNA (dsRNA) of cockroach period (Rm´per), timeless 1 (Rm´tim1), or cryptochrome 2 (Rm´cry2) we searched for essential components of the clock´s core negative feedback loop. Single injections of dsRNA of each clock gene into adult cockroaches successfully and permanently knocked down respective mRNA levels within ~two weeks deleting daytime-dependent mRNA rhythms for Rm´per and Rm´cry2. Rm´perRNAi or Rm´cry2RNAi affected total mRNA levels of both genes, while Rm´tim1 transcription was independent of both, also keeping rhythmic expression. Unexpectedly, circadian locomotor activity of most cockroaches remained rhythmic for each clock gene knockdown employed. It expressed weakened rhythms and unchanged periods for Rm´perRNAi and shorter periods for Rm´tim1RNAi and Rm´cry2RNAi.As a hypothesis of the cockroach´s molecular clockwork, a basic network of switched differential equations was developed to model the oscillatory behavior of clock cells expressing respective clock genes. Data were consistent with two synchronized main groups of coupled oscillator cells, a leading (morning) oscillator, or a lagging (evening) oscillator that couple via mutual inhibition. The morning oscillators express shorter, the evening oscillators longer endogenous periods based on core feedback loops with either PER, TIM1, or CRY2/PER complexes as dominant negative feedback of the clockwork. We hypothesize that dominant morning oscillator cells with shorter periods express PER, but not CRY2, or TIM1 as suppressor of clock gene expression, while two groups of evening oscillator cells with longer periods either comprise TIM1 or CRY2/PER suppressing complexes. Modelling suggests that there is an additional negative feedback next to Rm´PER in cockroach morning oscillator cells.eng
dcterms.accessRightsopen access
dcterms.creatorWerckenthin, Achim
dcterms.creatorHuber, Jannik
dcterms.creatorArnold, Thordis
dcterms.creatorKoziarek, Susanne
dcterms.creatorPlath, Marcus J. A.
dcterms.creatorPlath, Jenny Aino
dcterms.creatorStursberg, Olaf
dcterms.creatorHerzel, Hanspeter
dcterms.creatorStengl, Monika
dc.relation.doidoi:10.1371/journal.pone.0235930
dc.subject.swdTagesrhythmusger
dc.subject.swdBiorhythmusger
dc.subject.swdSchabenger
dc.subject.swdGenexpressionger
dc.subject.swdRNS-Interferenzger
dc.type.versionpublishedVersion
dcterms.source.identifierEISSN 1932-6203
dcterms.source.issueIssue 8
dcterms.source.journalPlos Oneeng
dcterms.source.pageinfoe0235930
dcterms.source.volumeVolume 15
kup.iskupfalse


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Except where otherwise noted, this item's license is described as Namensnennung 4.0 International