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Datum
2021-03-11Autor
Habenstein, JensSchmitt, FranziskaLiessem, SanderLy, AliceTrede, DennisWegener, ChristianPredel, ReinhardRössler, WolfgangNeupert, SusanneSchlagwort
570 Biowissenschaften, Biologie CataglyphisGehirnMatrix-unterstützte Laser-DesorptionNeuropeptideSoziale InsektenGenMetadata
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Aufsatz
Transcriptomic, peptidomic, and mass spectrometry imaging analysis of the brain in the ant Cataglyphis nodus
Zusammenfassung
Behavioral flexibility is an important cornerstone for the ecological success of animals. Social Cataglyphis nodus ants with their age-related polyethism characterized by age-related behavioral phenotypes represent a prime example for behavioral flexibility. We propose neuropeptides as powerful candidates for the flexible modulation of age-related behavioral transitions in individual ants. As the neuropeptidome of C. nodus was unknown, we collected a comprehensive peptidomic data set obtained by transcriptome analysis of the ants’ central nervous system combined with brain extract analysis by Q-Exactive Orbitrap mass spectrometry (MS) and direct tissue profiling of different regions of the brain by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS. In total, we identified 71 peptides with likely bioactive function, encoded on 49 neuropeptide-, neuropeptide-like, and protein hormone prepropeptide genes, including a novel neuropeptide-like gene (fliktin). We next characterized the spatial distribution of a subset of peptides encoded on 16 precursor proteins with high resolution by MALDI MS imaging (MALDI MSI) on 14 µm brain sections. The accuracy of our MSI data were confirmed by matching the immunostaining patterns for tachykinins with MSI ion images from consecutive brain sections. Our data provide a solid framework for future research into spatially resolved qualitative and quantitative peptidomic changes associated with stage-specific behavioral transitions and the functional role of neuropeptides in Cataglyphis ants.
Zitierform
In: Journal of Neurochemistry Volume 158 / Issue 2 (2021-03-11) , S. 391-412 ; eissn:1471-4159Förderhinweis
Gefördert im Rahmen des Projekts DEALZitieren
@article{doi:10.17170/kobra-202107294433,
author={Habenstein, Jens and Schmitt, Franziska and Liessem, Sander and Ly, Alice and Trede, Dennis and Wegener, Christian and Predel, Reinhard and Rössler, Wolfgang and Neupert, Susanne},
title={Transcriptomic, peptidomic, and mass spectrometry imaging analysis of the brain in the ant Cataglyphis nodus},
journal={Journal of Neurochemistry},
year={2021}
}
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2021-08-23T12:11:16Z 2021-08-23T12:11:16Z 2021-03-11 doi:10.17170/kobra-202107294433 http://hdl.handle.net/123456789/13151 Gefördert im Rahmen des Projekts DEAL eng Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ Catalyhis nodus brain MALDI imaging neuropeptides neuropeptidomics social insect transcriptomics 570 Transcriptomic, peptidomic, and mass spectrometry imaging analysis of the brain in the ant Cataglyphis nodus Aufsatz Behavioral flexibility is an important cornerstone for the ecological success of animals. Social Cataglyphis nodus ants with their age-related polyethism characterized by age-related behavioral phenotypes represent a prime example for behavioral flexibility. We propose neuropeptides as powerful candidates for the flexible modulation of age-related behavioral transitions in individual ants. As the neuropeptidome of C. nodus was unknown, we collected a comprehensive peptidomic data set obtained by transcriptome analysis of the ants’ central nervous system combined with brain extract analysis by Q-Exactive Orbitrap mass spectrometry (MS) and direct tissue profiling of different regions of the brain by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS. In total, we identified 71 peptides with likely bioactive function, encoded on 49 neuropeptide-, neuropeptide-like, and protein hormone prepropeptide genes, including a novel neuropeptide-like gene (fliktin). We next characterized the spatial distribution of a subset of peptides encoded on 16 precursor proteins with high resolution by MALDI MS imaging (MALDI MSI) on 14 µm brain sections. The accuracy of our MSI data were confirmed by matching the immunostaining patterns for tachykinins with MSI ion images from consecutive brain sections. Our data provide a solid framework for future research into spatially resolved qualitative and quantitative peptidomic changes associated with stage-specific behavioral transitions and the functional role of neuropeptides in Cataglyphis ants. open access Habenstein, Jens Schmitt, Franziska Liessem, Sander Ly, Alice Trede, Dennis Wegener, Christian Predel, Reinhard Rössler, Wolfgang Neupert, Susanne doi:10.1111/jnc.15346 Cataglyphis Gehirn Matrix-unterstützte Laser-Desorption Neuropeptide Soziale Insekten Gen publishedVersion eissn:1471-4159 Issue 2 Journal of Neurochemistry 391-412 Volume 158 false
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