Datum
2023-07-05Schlagwort
570 Biowissenschaften, Biologie 580 Pflanzen (Botanik) 590 Tiere (Zoologie) DeutschlandCuxhavenHamburgPollenDNA BarcodingHummelNahrungserwerbMetadata
Zur Langanzeige
Aufsatz
Pollen metabarcoding of museum specimens and recently collected bumblebees (Bombus) indicates foraging shifts
Zusammenfassung
Landscape changes, over time, lead to changes of floral resources available to pollinators, which in turn may result in the disappearance of ecologically specialized species. Here, we use pollen metabarcoding to infer historic and recent interactions between plants and bumblebees (Bombus). Bumblebees from Cuxhaven (Germany) were sampled from historical museum collections (1968/69) and in the field (2019). Pollen attached to their bodies was barcoded using multiple plant markers (ITS1, ITS2 and trnL-P6 loop). Our results show shifts in foraging habits between the historic and recent sampling periods, mostly determined by fewer Fabaceae interactions in 2019. The successful implementation of scalable molecular techniques for the analysis of historical pollen samples underscores the value of museum collections as a resource for biodiversity research. This study provides proof of concept of a comparative analysis of recent and historical pollination data. However, to ensure the robustness of our results, it is crucial to consider the broader methodology used. Our study found variation in the efficacy of the three plant barcoding markers. The ITS1 marker exhibited the highest species-level identification success, while the trnL-P6 loop demonstrated utility in amplifying degraded DNA across diverse plant families.
Zitierform
In: Metabarcoding and Metagenomics 7 (2023-07-05) , S. 89-119 ; eissn:2534-9708Förderhinweis
Gefördert durch den Publikationsfonds der Universität KasselZitieren
@article{doi:10.17170/kobra-202307218435,
author={Kolter, Andreas and Husemann, Martin and Podsiadlowski, Lars and Gemeinholzer, Birgit},
title={Pollen metabarcoding of museum specimens and recently collected bumblebees (Bombus) indicates foraging shifts},
journal={Metabarcoding and Metagenomics},
year={2023}
}
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2023-07-21T11:22:31Z 2023-07-21T11:22:31Z 2023-07-05 doi:10.17170/kobra-202307218435 http://hdl.handle.net/123456789/14907 Gefördert durch den Publikationsfonds der Universität Kassel eng Namensnennung 4.0 International http://creativecommons.org/licenses/by/4.0/ barcoding Bombus bumblebee Cuxhaven Hamburg ITS1 ITS ITS2 natural history collection plant metabarcoding pollen trnL-F P6eng 570 580 590 Pollen metabarcoding of museum specimens and recently collected bumblebees (Bombus) indicates foraging shifts Aufsatz Landscape changes, over time, lead to changes of floral resources available to pollinators, which in turn may result in the disappearance of ecologically specialized species. Here, we use pollen metabarcoding to infer historic and recent interactions between plants and bumblebees (Bombus). Bumblebees from Cuxhaven (Germany) were sampled from historical museum collections (1968/69) and in the field (2019). Pollen attached to their bodies was barcoded using multiple plant markers (ITS1, ITS2 and trnL-P6 loop). Our results show shifts in foraging habits between the historic and recent sampling periods, mostly determined by fewer Fabaceae interactions in 2019. The successful implementation of scalable molecular techniques for the analysis of historical pollen samples underscores the value of museum collections as a resource for biodiversity research. This study provides proof of concept of a comparative analysis of recent and historical pollination data. However, to ensure the robustness of our results, it is crucial to consider the broader methodology used. Our study found variation in the efficacy of the three plant barcoding markers. The ITS1 marker exhibited the highest species-level identification success, while the trnL-P6 loop demonstrated utility in amplifying degraded DNA across diverse plant families. open access Kolter, Andreas Husemann, Martin Podsiadlowski, Lars Gemeinholzer, Birgit doi:10.3897/mbmg.7.86883 Deutschland Cuxhaven Hamburg Pollen DNA Barcoding Hummel Nahrungserwerb publishedVersion eissn:2534-9708 Metabarcoding and Metagenomics 89-119 7 false e86883
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