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Datum
2024-03-22Autor
Leme Oliva, RebecaVogt, CarlaBublitz, Tábata AlineCamenzind, TessaDyckmans, JensJoergensen, Rainer GeorgSchlagwort
570 Biowissenschaften, Biologie AminozuckerZellwandMuraminsäureGlucosaminBioenergieMikroorganismusPolymereMetadata
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Aufsatz
Galactosamine and mannosamine are integral parts of bacterial and fungal extracellular polymeric substances
Zusammenfassung
Extracellular polymeric substances (EPS) are produced by microorganisms and interact to form a complex matrix called biofilm. In soils, EPS are important contributors to the microbial necromass and, thus, to soil organic carbon (SOC). Amino sugars (AS) are used as indicators for microbial necromass in soil, although the origin of galactosamine and mannosamine is largely unknown. However, indications exist that they are part of EPS. In this study, two bacteria and two fungi were grown in starch medium either with or without a quartz matrix to induce EPS production. Each culture was separated in two fractions: one that directly underwent AS extraction (containing AS from both biomass and EPS), and another that first had EPS extracted, followed then by AS determination (exclusively containing AS from EPS). We did not observe a general effect of the quartz matrix neither of microbial type on AS production. The quantified amounts of galactosamine and mannosamine in the EPS fraction represented on average 100% of the total amounts of these two AS quantified in cell cultures, revealing they are integral parts of the biofilm. In contrast, muramic acid and glucosamine were also quantified in the EPS, but with much lower contribution rates to total AS production, of 18% and 33%, respectively, indicating they are not necessarily part of EPS. Our results allow a meaningful ecological interpretation of mannosamine and galactosamine data in the future as indicators of microbial EPS, and also attract interest of future studies to investigate the role of EPS to SOC and its dynamics.
Zitierform
In: ISME Communications Volume 4 / Issue 1 (2024-03-22) eissn:2730-6151Förderhinweis
Gefördert durch den Publikationsfonds der Universität KasselZitieren
@article{doi:10.17170/kobra-2024052410202,
author={Leme Oliva, Rebeca and Vogt, Carla and Bublitz, Tábata Aline and Camenzind, Tessa and Dyckmans, Jens and Joergensen, Rainer Georg},
title={Galactosamine and mannosamine are integral parts of bacterial and fungal extracellular polymeric substances},
journal={ISME Communications},
year={2024}
}
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2024-05-24T12:49:49Z 2024-05-24T12:49:49Z 2024-03-22 doi:10.17170/kobra-2024052410202 http://hdl.handle.net/123456789/15778 Gefördert durch den Publikationsfonds der Universität Kassel eng Namensnennung 4.0 International http://creativecommons.org/licenses/by/4.0/ EPS amino sugars cell-wall components muramic acid glucosamine 570 Galactosamine and mannosamine are integral parts of bacterial and fungal extracellular polymeric substances Aufsatz Extracellular polymeric substances (EPS) are produced by microorganisms and interact to form a complex matrix called biofilm. In soils, EPS are important contributors to the microbial necromass and, thus, to soil organic carbon (SOC). Amino sugars (AS) are used as indicators for microbial necromass in soil, although the origin of galactosamine and mannosamine is largely unknown. However, indications exist that they are part of EPS. In this study, two bacteria and two fungi were grown in starch medium either with or without a quartz matrix to induce EPS production. Each culture was separated in two fractions: one that directly underwent AS extraction (containing AS from both biomass and EPS), and another that first had EPS extracted, followed then by AS determination (exclusively containing AS from EPS). We did not observe a general effect of the quartz matrix neither of microbial type on AS production. The quantified amounts of galactosamine and mannosamine in the EPS fraction represented on average 100% of the total amounts of these two AS quantified in cell cultures, revealing they are integral parts of the biofilm. In contrast, muramic acid and glucosamine were also quantified in the EPS, but with much lower contribution rates to total AS production, of 18% and 33%, respectively, indicating they are not necessarily part of EPS. Our results allow a meaningful ecological interpretation of mannosamine and galactosamine data in the future as indicators of microbial EPS, and also attract interest of future studies to investigate the role of EPS to SOC and its dynamics. open access Leme Oliva, Rebeca Vogt, Carla Bublitz, Tábata Aline Camenzind, Tessa Dyckmans, Jens Joergensen, Rainer Georg 7 Seiten doi:10.1093/ismeco/ycae038 Aminozucker Zellwand Muraminsäure Glucosamin Bioenergie Mikroorganismus Polymere publishedVersion eissn:2730-6151 Issue 1 ISME Communications Volume 4 false ycae038
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