Date
2024-07-20Metadata
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Aufsatz
This work was funded by the Research Association of the German Food Industry (FEI), supported by the German Ministry of Economics and Technology (via AiF) (Project No. 21630N).
Curse or blessing: Growth- and laccase-modulating properties of polyphenols and their oxidized derivatives on Botrytis cinerea
Abstract
Infection of grapevines with the grey mold pathogen Botrytis cinerea results in severe problems for winemakers worldwide. Browning of wine is caused by the laccase-mediated oxidation of polyphenols. In the last decades, Botrytis management has become increasingly difficult due to the rising number of resistances and the genetic variety of Botrytis strains. During the search for sustainable fungicides, polyphenols showed great potential to inhibit fungal growth. The present study revealed two important aspects regarding the effects of grape-specific polyphenols and their polymerized oxidation products on Botrytis wild strains. On the one hand, laccase-mediated oxidized polyphenols, which resemble the products found in infected grapes, showed the same potential for inhibition of growth and laccase activity, but differed from their native forms. On the other hand, the impact of phenolic compounds on mycelial growth is not correlated to the effect on laccase activity. Instead, mycelial growth and relative specific laccase activity appear to be modulated independently. All phenolic compounds showed not only inhibitory but also inductive effects on fungal growth and/or laccase activity, an observation which is reported for the first time. The simultaneous inhibition of growth and laccase activity demonstrated may serve as a basis for the development of a natural botryticide. Yet, the results showed considerable differences between genetically distinguishable strains, impeding the use of a specific phenolic compound against the genetic variety of wild strains. The present findings might have important implications for future understanding of Botrytis cinerea infections and sustainable Botrytis management including the role of polyphenols.
Sponsorship
Gefördert im Rahmen des Projekts DEALThis work was funded by the Research Association of the German Food Industry (FEI), supported by the German Ministry of Economics and Technology (via AiF) (Project No. 21630N).
Citation
@article{doi:10.17170/kobra-2024081610682,
author={Umberath, Kim Marie and Mischke, Anna and Caspers-Weiffenbach, Rita and Backmann, Louis and Scharfenberger-Schmeer, Maren and Wegmann-Herr, Pascal and Schieber, Andreas and Weber, Fabian},
title={Curse or blessing: Growth- and laccase-modulating properties of polyphenols and their oxidized derivatives on Botrytis cinerea},
journal={Food Research International},
year={2024}
}
0500 Oax
0501 Text $btxt$2rdacontent
0502 Computermedien $bc$2rdacarrier
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1500 1/eng
2050 ##0##http://hdl.handle.net/123456789/16020
3000 Umberath, Kim Marie
3010 Mischke, Anna
3010 Caspers-Weiffenbach, Rita
3010 Backmann, Louis
3010 Scharfenberger-Schmeer, Maren
3010 Wegmann-Herr, Pascal
3010 Schieber, Andreas
3010 Weber, Fabian
4000 Curse or blessing: Growth- and laccase-modulating properties of polyphenols and their oxidized derivatives on Botrytis cinerea / Umberath, Kim Marie
4030
4060 Online-Ressource
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4170
5550 {{Botrytis cinerea}}
5550 {{Weinrebe}}
5550 {{Schimmel}}
5550 {{Laccase}}
5550 {{Phenole}}
5550 {{Polyphenole}}
5550 {{Wachstumshemmung}}
5550 {{Fungizid}}
7136 ##0##http://hdl.handle.net/123456789/16020
<resource xsi:schemaLocation="http://datacite.org/schema/kernel-2.2 http://schema.datacite.org/meta/kernel-2.2/metadata.xsd"> 2024-09-04T10:26:11Z 2024-09-04T10:26:11Z 2024-07-20 doi:10.17170/kobra-2024081610682 http://hdl.handle.net/123456789/16020 Gefördert im Rahmen des Projekts DEAL This work was funded by the Research Association of the German Food Industry (FEI), supported by the German Ministry of Economics and Technology (via AiF) (Project No. 21630N). eng Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ Botrytis cinerea grapevine grey mold laccase phenolic compounds polymerized polyphenols growth inhibition sustainable fungicides 570 580 Curse or blessing: Growth- and laccase-modulating properties of polyphenols and their oxidized derivatives on Botrytis cinerea Aufsatz Infection of grapevines with the grey mold pathogen Botrytis cinerea results in severe problems for winemakers worldwide. Browning of wine is caused by the laccase-mediated oxidation of polyphenols. In the last decades, Botrytis management has become increasingly difficult due to the rising number of resistances and the genetic variety of Botrytis strains. During the search for sustainable fungicides, polyphenols showed great potential to inhibit fungal growth. The present study revealed two important aspects regarding the effects of grape-specific polyphenols and their polymerized oxidation products on Botrytis wild strains. On the one hand, laccase-mediated oxidized polyphenols, which resemble the products found in infected grapes, showed the same potential for inhibition of growth and laccase activity, but differed from their native forms. On the other hand, the impact of phenolic compounds on mycelial growth is not correlated to the effect on laccase activity. Instead, mycelial growth and relative specific laccase activity appear to be modulated independently. All phenolic compounds showed not only inhibitory but also inductive effects on fungal growth and/or laccase activity, an observation which is reported for the first time. The simultaneous inhibition of growth and laccase activity demonstrated may serve as a basis for the development of a natural botryticide. Yet, the results showed considerable differences between genetically distinguishable strains, impeding the use of a specific phenolic compound against the genetic variety of wild strains. The present findings might have important implications for future understanding of Botrytis cinerea infections and sustainable Botrytis management including the role of polyphenols. open access Umberath, Kim Marie Mischke, Anna Caspers-Weiffenbach, Rita Backmann, Louis Scharfenberger-Schmeer, Maren Wegmann-Herr, Pascal Schieber, Andreas Weber, Fabian doi:10.1016/j.foodres.2024.114782 Project No. 21630N Botrytis cinerea Weinrebe Schimmel Laccase Phenole Polyphenole Wachstumshemmung Fungizid publishedVersion eissn:1873-7145 Food Research International Volume 192 false </resource>
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