Aufsatz
Inoculum microbial mass is negatively related to microbial yield and positively to methane yield in vitro
Abstract
Ruminal microbes catabolise feed carbohydrates mainly into SCFA, methane (CH₄), and carbon dioxide (CO₂), with predictable relationships between fermentation end products and net microbial increase. We used a closed in vitro batch culture system, incubating grass and maize silages, and measured total gas production at 8 and 24 h, as well as the truly degraded substrate, the net production of SCFA, CH₄, and microbial biomass at 24 h, and investigated the impact of silage type and inoculum microbial mass on fermentation direction. Net microbial yield was negatively correlated with total gas at 8 h (P < 0•001), but not at 24 h (P = 0•052), and negatively correlated with CH₄ production (P < 0•001). Higher initial inoculum microbial mass was related to a lower net microbial yield (P < 0•001) but a higher CH₄ production (P < 0•001). A significant difference between grass silage and maize silage was detected within the context of these relationships (P < 0•050). The metabolic hydrogen (2H) recovery was 102.8 ± 12.3 % for grass silages and 118.8 ± 13.3% for maize silages. Overall, grass silages favoured more substrate conversion to microbial biomass and less to fermentation end products than maize silage. Lower inoculum microbial mass facilitated more microbial growth and, because of the 2H sink by microbial synthesis, decreased CH₄ production.
Citation
In: Journal of Nutritional Science : JNS Volume 13 (2024-09-20) eissn:2048-6790Sponsorship
Gefördert im Rahmen des Projekts DEALCitation
@article{doi:10.17170/kobra-2024101810958,
author={Zhang, Xiaoyu and Klevenhusen, Fenja and Sünder, Angela and Clauss, Marcus and Hummel, Jürgen},
title={Inoculum microbial mass is negatively related to microbial yield and positively to methane yield in vitro},
journal={Journal of Nutritional Science : JNS},
year={2024}
}
0500 Oax 0501 Text $btxt$2rdacontent 0502 Computermedien $bc$2rdacarrier 1100 2024$n2024 1500 1/eng 2050 ##0##http://hdl.handle.net/123456789/16092 3000 Zhang, Xiaoyu 3010 Klevenhusen, Fenja 3010 Sünder, Angela 3010 Clauss, Marcus 3010 Hummel, Jürgen 4000 Inoculum microbial mass is negatively related to microbial yield and positively to methane yield in vitro / Zhang, Xiaoyu 4030 4060 Online-Ressource 4085 ##0##=u http://nbn-resolving.de/http://hdl.handle.net/123456789/16092=x R 4204 \$dAufsatz 4170 5550 {{Mikrobieller Abbau}} 5550 {{Methan}} 5550 {{Stöchiometrie}} 7136 ##0##http://hdl.handle.net/123456789/16092
2024-10-18T10:50:43Z 2024-10-18T10:50:43Z 2024-09-20 doi:10.17170/kobra-2024101810958 http://hdl.handle.net/123456789/16092 Gefördert im Rahmen des Projekts DEAL eng Namensnennung 4.0 International http://creativecommons.org/licenses/by/4.0/ Degraded substrate partitioning Initial microbial mass Metabolic hydrogen Methane Stoichiometric relationship 570 Inoculum microbial mass is negatively related to microbial yield and positively to methane yield in vitro Aufsatz Ruminal microbes catabolise feed carbohydrates mainly into SCFA, methane (CH₄), and carbon dioxide (CO₂), with predictable relationships between fermentation end products and net microbial increase. We used a closed in vitro batch culture system, incubating grass and maize silages, and measured total gas production at 8 and 24 h, as well as the truly degraded substrate, the net production of SCFA, CH₄, and microbial biomass at 24 h, and investigated the impact of silage type and inoculum microbial mass on fermentation direction. Net microbial yield was negatively correlated with total gas at 8 h (P < 0•001), but not at 24 h (P = 0•052), and negatively correlated with CH₄ production (P < 0•001). Higher initial inoculum microbial mass was related to a lower net microbial yield (P < 0•001) but a higher CH₄ production (P < 0•001). A significant difference between grass silage and maize silage was detected within the context of these relationships (P < 0•050). The metabolic hydrogen (2H) recovery was 102.8 ± 12.3 % for grass silages and 118.8 ± 13.3% for maize silages. Overall, grass silages favoured more substrate conversion to microbial biomass and less to fermentation end products than maize silage. Lower inoculum microbial mass facilitated more microbial growth and, because of the 2H sink by microbial synthesis, decreased CH₄ production. open access Zhang, Xiaoyu Klevenhusen, Fenja Sünder, Angela Clauss, Marcus Hummel, Jürgen 9 Seiten doi:10.1017/jns.2024.37 Mikrobieller Abbau Methan Stöchiometrie publishedVersion eissn:2048-6790 Journal of Nutritional Science : JNS Volume 13 false e44
The following license files are associated with this item: