Aufsatz
The project receives funding via the Waldklimafonds (WKF) funded by the German Federal Ministry of Food and Agriculture (BMEL) and Federal Ministry for the Environment, Nature Conservation, Nuclear Safety and Consumer Protection (BMUV) administrated by the Agency for Renewable Resources (FNR) under grant agreement No. 2219WK22D4.
Fungi associated with fine roots of Fraxinus excelsior affected by ash dieback detected by next-generation sequencing
Abstract
The decline of European ash by dieback caused by Hymenoscyphus fraxineus together with stem collar necroses and rots caused by various fungi has been investigated intensively during the last years. Nevertheless, hitherto nearly nothing is known about the species diversity of the fungal rhizobiome of ash trees. Here we investigated the fine roots of affected ash trees on 15 sampling sites in 6 federal countries of Germany. Fine-root samples have been treated in three different sample regimes each as root-adhering soil, unsterilized fine roots and sterilized fine roots. The samples of trees in sampling sites were pooled to get an overview of the species-richness in the area. The next-generation sequencing platform Oxford Nanopore MinION was used to sequence the entire ITS of pooled probes. Most abundant phyla in all samples were the Basidiomycota and Ascomycota. Species richness in sterilized roots was significantly different from unsterilized roots and root-adhering soil. Surprisingly most abundant genera in sterilized roots were the genera Mycena, Mycenella and Delicatula, all of them agaricoids with saprophytic lifestyle. Eleven genera of Glomeromycota have been detected in various abundances, whereas the detection of H. fraxineus was neglectable.
Citation
In: Journal of Plant Diseases and Protection Volume 131 / Issue 5 (2024-05-04) , S. 1373-1385 ; eissn:1861-3837Sponsorship
Gefördert im Rahmen des Projekts DEALThe project receives funding via the Waldklimafonds (WKF) funded by the German Federal Ministry of Food and Agriculture (BMEL) and Federal Ministry for the Environment, Nature Conservation, Nuclear Safety and Consumer Protection (BMUV) administrated by the Agency for Renewable Resources (FNR) under grant agreement No. 2219WK22D4.
Citation
@article{doi:10.17170/kobra-2024103111039,
author={Lysenko, Ludmila and Griem, Erik and Wagener, Patrick and Langer, Ewald Johannes},
title={Fungi associated with fine roots of Fraxinus excelsior affected by ash dieback detected by next-generation sequencing},
journal={Journal of Plant Diseases and Protection},
year={2024}
}
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2024-10-31T09:18:16Z 2024-10-31T09:18:16Z 2024-05-04 doi:10.17170/kobra-2024103111039 http://hdl.handle.net/123456789/16129 Gefördert im Rahmen des Projekts DEAL The project receives funding via the Waldklimafonds (WKF) funded by the German Federal Ministry of Food and Agriculture (BMEL) and Federal Ministry for the Environment, Nature Conservation, Nuclear Safety and Consumer Protection (BMUV) administrated by the Agency for Renewable Resources (FNR) under grant agreement No. 2219WK22D4. eng Namensnennung 4.0 International http://creativecommons.org/licenses/by/4.0/ demonstration project European ash dieback Fraxinus excelsior fine roots mycobiome next-generation sequencing Oxford Nanopore MinION Decona Microeco PipeCraft2 500 570 Fungi associated with fine roots of Fraxinus excelsior affected by ash dieback detected by next-generation sequencing Aufsatz The decline of European ash by dieback caused by Hymenoscyphus fraxineus together with stem collar necroses and rots caused by various fungi has been investigated intensively during the last years. Nevertheless, hitherto nearly nothing is known about the species diversity of the fungal rhizobiome of ash trees. Here we investigated the fine roots of affected ash trees on 15 sampling sites in 6 federal countries of Germany. Fine-root samples have been treated in three different sample regimes each as root-adhering soil, unsterilized fine roots and sterilized fine roots. The samples of trees in sampling sites were pooled to get an overview of the species-richness in the area. The next-generation sequencing platform Oxford Nanopore MinION was used to sequence the entire ITS of pooled probes. Most abundant phyla in all samples were the Basidiomycota and Ascomycota. Species richness in sterilized roots was significantly different from unsterilized roots and root-adhering soil. Surprisingly most abundant genera in sterilized roots were the genera Mycena, Mycenella and Delicatula, all of them agaricoids with saprophytic lifestyle. Eleven genera of Glomeromycota have been detected in various abundances, whereas the detection of H. fraxineus was neglectable. open access Lysenko, Ludmila Griem, Erik Wagener, Patrick Langer, Ewald Johannes doi:10.1007/s41348-024-00923-z grant agreement No. 2219WK22D4 Esche Pilze Wurzel Sequenzanalyse <Chemie> publishedVersion eissn:1861-3837 Issue 5 Journal of Plant Diseases and Protection 1373-1385 Volume 131
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