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Dissertation
Synthese von Ester- und Etherlipiden durch die GPAT/AGPAT-Proteinfamilie und Lipin in Dictyostelium
(2021-05-12)
The synthesis of glycerolipids starts with the acylation of lipid backbones. Two structurally different glycerolipids emerge in the cell: ether-lipids and ester-lipids. In the case of ether-lipids the first fatty acid is substituted with a fatty alcohol inside the peroxisome. By the characterization of the protein FARAT in this thesis, a key enzyme in etherlipid-synthesis was identified for D. discoideum. FARAT shows a peroxisomal localization and is in principle able to do two jobs. Firstly, it initiates the peroxisomal ...
Dissertation
Investigation of the functional synergy and involvement of different tRNA modifications in translational control
(2020-07-02)
Transfer RNAs are essential adapter molecules employed in the translational process and are highly modified all over their structure. Specific modification of the four canonical ribonucleotides (A, U, C, G) is accomplished by various proteins and/or protein complexes, ranging from simple isomerisations (e.g. Ψ) and methylations (e.g. m5C) up to complex chemical structures (e.g. mcm5s2U, ct6A). The functions of tRNA modifications are linked to the specific position and may influence translation by supporting the native ...
Dissertation
The tRNA binding protein Kti12 and its role in the regulation of Elongator complex
(2020-06-08)
[Summary] Kti12 is a conserved eukaryotic protein showing sequence homology to O-phosphoseryltRNA kinase (Pstk), which is required for the synthesis of selenocysteine tRNA. Saccharomyces cerevisiae Kti12 was originally discovered in a genetic screening for resistance against zymocin, a protein toxin produced by Kluyveromyces lactis. The cytotoxicity of zymocin lies in its anticodon nuclease activity, causing tRNA depletion and cell death in sensitive yeast cells. It recognizes and cleaves a particular wobble uridine ...