Search
Now showing items 21-25 of 25
Aufsatz
![Open Access](/themes/Mirage2/images/Open_Access_logo_PLoS_transparent.svg)
Yeast gene KTI13 (alias DPH8) operates in the initiation step of diphthamide synthesis on elongation factor 2
(2023-08-08)
In yeast, Elongator-dependent tRNA modifications are regulated by the Kti11•Kti13 dimer and hijacked for cell killing by zymocin, a tRNase ribotoxin. Kti11 (alias Dph3) also controls modification of elongation factor 2 (EF2) with diphthamide, the target for lethal ADP-ribosylation by diphtheria toxin (DT). Diphthamide formation on EF2 involves four biosynthetic steps encoded by the DPH1-DPH7 network and an ill-defined KTI13 function. On further examining the latter gene in yeast, we found that kti13Δ null-mutants ...
Aufsatz
![Open Access](/themes/Mirage2/images/Open_Access_logo_PLoS_transparent.svg)
Philip Charles Brookes, 23 March 1951 to 28 September 2023
(2023-12-14)
Obituary to Philip Charles Brookes (1951-2023).
Aufsatz
![Open Access](/themes/Mirage2/images/Open_Access_logo_PLoS_transparent.svg)
Lipidome remodeling in response to nutrient replenishment requires the tRNA modifier Deg1/Pus3 in yeast
(2023-10-21)
In the yeast Saccharomyces cerevisiae, the absence of the pseudouridine synthase Pus3/Deg1, which modifies tRNA positions 38 and 39, results in increased lipid droplet (LD) content and translational defects. In addition, starvation-like transcriptome alterations and induced protein aggregation were observed. In this study, we show that the deg1 mutant increases specific misreading errors. This could lead to altered expression of the main regulators of neutral lipid synthesis which are the acetyl-CoA carboxylase (Acc1), ...
Aufsatz
![Open Access](/themes/Mirage2/images/Open_Access_logo_PLoS_transparent.svg)
Anthropogenic and natural disturbances increase local genetic diversity in an early spring geophyte (Ficaria verna Huds)
(2023-12-26)
The tetraploid Ficaria verna is a common spring geophyte in central Europe and is considered invasive in the USA and Canada. It is considered an almost seed-sterile taxon, relying on vegetative reproduction by underground tubers and aerial bulbils. Recent studies have revealed high levels of population genetic diversity in F. verna, raising the question of how genetic diversity is maintained and which factors may be responsible for the observed patterns. Polymorphic nuclear microsatellite markers were established to ...
Aufsatz
![Open Access](/themes/Mirage2/images/Open_Access_logo_PLoS_transparent.svg)
Optimization of the bicinchoninic acid assay for quantifying carbohydrates of soil extracellular polymeric substances
(2023-12-23)
Background and aims
The bicinchoninic acid (BCA) method was not yet applied on soil extracts of extracellular polymeric substances (EPS) to quantify polysaccharides, although this might be possible by introducing a cleavage step to produce monosaccharides. A pre-extraction with CaCl₂ to remove interfering substances is usually performed before extracting EPS. The main objective of this study was to optimize the BCA assay for total carbohydrates quantification by applying a hydrolysis step to the EPS extracts, while ...