Suche
Anzeige der Dokumente 51-60 von 136
Aufsatz
The Thermodynamic Stability of Membrane Proteins in Micelles and Lipid Bilayers Investigated with the Ferrichrom Receptor FhuA
(2022-05-13)
Extraction of integral membrane proteins into detergents for structural and functional studies often leads to a strong loss in protein stability. The impact of the lipid bilayer on the thermodynamic stability of an integral membrane protein in comparison to its solubilized form in detergent was examined and compared for FhuA from Escherichia coli and for a mutant, FhuAΔ5-160, lacking the N-terminal cork domain. Urea-induced unfolding was monitored by fluorescence spectroscopy to determine the effective free energies ...
Aufsatz
A comparison of online and live training of livestock farmers for an on-farm self-assessment of animal welfare
(2022-11-14)
One approach to strengthening the involvement of farmers or stockpersons in the evaluation and improvement of animal welfare is the implementation of an on-farm self-assessment. A valid comparison of the results with reference values, between or within farms, requires that training of the farmers and reliability testing have taken place. We investigated two different training methods (online vs. live) with a total of 146 livestock farmers from farms with dairy cows and calves, beef cattle, sows and suckling piglets, ...
Aufsatz
Spatial and Temporal Variations of Stable Isotopes in Precipitation in the Mountainous Region, North Hesse
(2022-12-01)
Patterns of stable isotopes of water (¹⁸O and ²H) in precipitation have been used as tracers for analyzing environmental processes which can be changed by factors such as the topography or meteorological variables. In this study, we investigated the isotopic data in precipitation for one year in the low mountain range of North Hesse, Germany, and analyzed mainly for altitude, rainfall amount, and air temperature effects on a regional scale. The results indicate that the isotopic composition expressed an altitude ...
Aufsatz
The potential of metabarcoding plant components of Malaise trap samples to enhance knowledge of plant-insect interactions
(2022-07-22)
The worldwide rapid declines in insect and plant abundance and diversity that have occurred in the past decades have gained public attention and demand for political actions to counteract these declines are growing. Rapid large-scale biomonitoring can aid in observing these changes and provide information for decisions for land management and species protection. Malaise traps have long been used for insect sampling and when insects are captured in these traps, they carry traces of plants they have visited on the body ...
Aufsatz
Angebot, Nutzung und Ertrag von Konzeptwechseltexten zu Neuromythen bei angehenden Biologielehrkräften
(2021-04-27)
Lehrkräftebildung hat das Ziel, Studierende zu Fachleuten für das Lehren und Lernen auszubilden. Empirische Studien zeigen jedoch sowohl bei angehenden als auch praktizierenden Lehrkräften eine hohe Zustimmung zu Fehlvorstellungen zum Thema Gehirn und Lernen – sogenannten Neuromythen. In der vorliegenden Studie wird in einem Mixed-Model-Design (N = 40) mit quantitativen und qualitativen Forschungsmethoden und vor dem theoretischen Hintergrund eines Angebots-Nutzungs-Modells untersucht, inwiefern sich durch eine ...
Aufsatz
Phospholipid fatty acids in soil—drawbacks and future prospects
(2021-12-21)
The current opinion and position paper highlights (1) correct assignation of indicator phospholipid fatty acids (PLFA), (2) specificity and recycling of PLFA in microorganisms, and (3) complete extraction and detection of PLFA. The straight-chain PLFA 14:0, 15:0, 16:0, and 17:0 occur in all microorganisms, i.e., also in fungi and not only in bacteria. If the phylum Actinobacteria is excluded from the group of Gram-positive bacteria, all remaining bacteria belong to the bacterial phylum Firmicutes, which should be ...
Aufsatz
tRNA anticodon loop modifications ensure protein homeostasis and cell morphogenesis in yeast
(2016-08-05)
Using budding yeast, we investigated a negative interaction network among genes for tRNA modifications previously implicated in anticodon-codon interaction: 5-methoxy-carbonyl-methyl-2-thio-uridine (mcm5s2U34: ELP3, URM1), pseudouridine (Ψ38/39: DEG1) and cyclic N6-threonyl-carbamoyl-adenosine (ct6A37: TCD1). In line with functional cross talk between these modifications, we find that combined removal of either ct6A37 or Ψ38/39 and mcm5U34 or s2U34 results in morphologically altered cells with synthetic growth defects. ...
Aufsatz
Phylogenetic and morphological studies in Xylodon (Hymenochaetales, Basidiomycota) with the addition of four new species
(2019-02-28)
Xylodon (Hymenochaetales, Basidiomycota) is the largest segregate genus of Hyphodontia s.l. Based on molecular and morphological data, 77 species are accepted in Xylodon to date. Phylogenetic analyses of ITS and 28S sequences, including 38 new ITS and 20 28S sequences of Xylodon species, revealed four species new to science. The new taxa X. exilis, X. filicinus, X. follis and X. pseudolanatus from Taiwan, Nepal, Réunion, Belize, and USA are described and illustrated. In addition, species concepts for Odontia vesiculosa ...
Aufsatz
Short-spored Subulicystidium (Trechisporales, Basidiomycota): high morphological diversity and only partly clear species boundaries
(2018-06-27)
Diversity of corticioid fungi (resupinate Basidiomycota), especially outside the northern temperate climatic zone, remains poorly explored. Furthermore, most of the known species are delimited by morphological concepts only and, not rarely, these concepts are too broad and need to be tested by molecular tools. For many decades, the delimitation of species in the genus Subulicystidium (Hydnodontaceae, Trechisporales) was a challenge for mycologists. The presence of numerous transitional forms as to basidiospore size ...
Aufsatz
Pre-service Science Teachers’ Neuroscience Literacy: Neuromyths and a Professional Understanding of Learning and Memory
(2019-02-14)
Transferring current research findings on the topic of learning and memory to “brain-based” learning in schools is of great interest among teachers. However, numerous international studies demonstrate that both pre-service and in-service teachers do not always succeed. Instead, they transfer numerous misconceptions about neuroscience, known as neuromyths, into pedagogical practice. As a result, researchers call for more neuroscience in teacher education in order to create a professional understanding of learning and ...