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2018-07-03
Author
Aakhte, MostafaAkhlaghi, Ehsan A.Müller, Hans-Arno J.
Subject
570  Life sciences; biology 
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urn:nbn:de:hebis:34-2018082156257

doi:10.1038/s41598-018-28389-8
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Aufsatz

SSPIM: a beam shaping toolbox for structured selective plane illumination microscopy

Abstract
Selective plane illumination microscopy (SPIM) represents a preferred method in dynamic tissue imaging, because it combines high spatiotemporal resolution with low phototoxicity. The OpenSPIM system was developed to provide an accessible and flexible microscope set-up for non-specialist users. Here, we report Structured SPIM (SSPIM), which offers an open-source, user-friendly and compact toolbox for beam shaping to be applied within the OpenSPIM platform. SSPIM is able to generate digital patterns for a wide range of illumination beams including static and spherical Gaussian beams, Bessel beams and Airy beams by controlling the pattern of a Spatial Light Modulator (SLM). In addition, SSPIM can produce patterns for structured illumination including incoherent and coherent array beams and tiling for all types of the supported beams. We describe the workflow of the toolbox and demonstrate its application by comparing experimental data with simulation results for a wide range of illumination beams. Finally, the capability of SSPIM is investigated by 3D imaging of Drosophila embryos using scanned Gaussian, Bessel and array beams. SSPIM provides an accessible toolbox to generate and optimize the desired beam patterns and helps adapting the OpenSPIM system towards a wider range of biological samples.
Citation
In: Scientific reports. - [London] : Springer Nature Limited. - (2018)8:10067
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Gefördert durch den Publikationsfonds der Universität Kassel
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Publikationen  (Fachgebiet Entwicklungsgenetik)
Artikel  (Publikationen im Open Access gefördert durch die UB)
Citation
@article{urn:nbn:de:hebis:34-2018082156257,
   author={Aakhte, Mostafa and Akhlaghi, Ehsan A. and Müller, Hans-Arno J.},
   title={SSPIM: a beam shaping toolbox for structured selective plane illumination microscopy},
   year={2018}
}
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3010 Müller, Hans-Arno J.
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2018-08-21T10:57:22Z
2018-08-21T10:57:22Z
2018-07-03
2045-2322
urn:nbn:de:hebis:34-2018082156257
http://hdl.handle.net/123456789/2018082156257
Gef&ouml;rdert durch den Publikationsfonds der Universit&auml;t Kassel
eng
Urheberrechtlich gesch&uuml;tzt
https://rightsstatements.org/page/InC/1.0/
570
SSPIM: a beam shaping toolbox for structured selective plane illumination microscopy
Aufsatz
Selective plane illumination microscopy (SPIM) represents a preferred method in dynamic tissue imaging, because it combines high spatiotemporal resolution with low phototoxicity. The OpenSPIM system was developed to provide an accessible and flexible microscope set-up for non-specialist users. Here, we report Structured SPIM (SSPIM), which offers an open-source, user-friendly and compact toolbox for beam shaping to be applied within the OpenSPIM platform. SSPIM is able to generate digital patterns for a wide range of illumination beams including static and spherical Gaussian beams, Bessel beams and Airy beams by controlling the pattern of a Spatial Light Modulator (SLM). In addition, SSPIM can produce patterns for structured illumination including incoherent and coherent array beams and tiling for all types of the supported beams. We describe the workflow of the toolbox and demonstrate its application by comparing experimental data with simulation results for a wide range of illumination beams. Finally, the capability of SSPIM is investigated by 3D imaging of Drosophila embryos using scanned Gaussian, Bessel and array beams. SSPIM provides an accessible toolbox to generate and optimize the desired beam patterns and helps adapting the OpenSPIM system towards a wider range of biological samples.
open access
In: Scientific reports. - [London] : Springer Nature Limited. - (2018)8:10067
Aakhte, Mostafa
Akhlaghi, Ehsan A.
M&uuml;ller, Hans-Arno J.
doi:10.1038/s41598-018-28389-8
</resource>

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Urheberrechtlich geschützt