Distribution and daily oscillation of GABA in the circadian system of the cockroach Rhyparobia maderae

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dc.date.accessioned2022-02-14T16:33:32Z
dc.date.available2022-02-14T16:33:32Z
dc.date.issued2021-09-29
dc.description.sponsorshipGefördert im Rahmen des Projekts DEALger
dc.description.sponsorshipDeutsche Forschungsgemeinschaft. Grant Numbers: HO 950/26-1, STE 531/26-1, STE 531/27-1
dc.identifierdoi:10.17170/kobra-202202015682
dc.identifier.urihttp://hdl.handle.net/123456789/13614
dc.language.isoengeng
dc.relation.doidoi:10.1002/cne.25244
dc.relation.projectidGrant Numbers: HO 950/26-1, STE 531/26-1, STE 531/27-1
dc.rightsNamensnennung-Nicht-kommerziell 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/*
dc.subjectaccessory medullaeng
dc.subjectcircadian rhythmseng
dc.subjectglutamic acid decarboxylaseeng
dc.subjectimmunocytochemistryeng
dc.subjectinsect braineng
dc.subject.ddc570
dc.subject.swdKüchenschabeger
dc.subject.swdTagesrhythmusger
dc.subject.swdKnochenmarkger
dc.subject.swdAminobuttersäure <gamma->ger
dc.subject.swdGlutamat-Decarboxylaseger
dc.subject.swdImmuncytochemieger
dc.subject.swdInsektenger
dc.subject.swdGehirnger
dc.titleDistribution and daily oscillation of GABA in the circadian system of the cockroach Rhyparobia maderaeeng
dc.typeAufsatz
dc.type.versionpublishedVersion
dcterms.abstractGamma-aminobutyric acid (GABA) is the prevalent inhibitory neurotransmitter in nervous systems promoting sleep in both mammals and insects. In the Madeira cockroach, sleep-wake cycles are controlled by a circadian clock network in the brain's optic lobes, centered in the accessory medulla (AME) with its innervating pigment-dispersing factor (PDF) expressing clock neurons at the anterior-ventral rim of the medulla. GABA is present in cell clusters that innervate different circuits of the cockroach's AME clock, without colocalizing in PDF clock neurons. Physiological, immunohistochemical, and behavioral assays provided evidence for a role of GABA in light entrainment, possibly via the distal tract that connects the AME's glomeruli to the medulla. Furthermore, GABA was implemented in clock outputs to multiple effector systems in optic lobe and midbrain. Here, GABAergic brain circuits were analyzed further, focusing on the circadian system in search for sleep/wake controlling brain circuits. All GABA-immunoreactive neurons of the cockroach brain were also stained with an antiserum against the GABA-synthesizing enzyme glutamic acid decarboxylase. We found strong overlap of the distribution of GABA-immunoreactive networks with PDF clock networks in optic lobes and midbrain. Neurons in five of the six soma groups that innervate the clock exhibited GABA immunoreactivity. The intensity of GABA immunoreactivity in the distal tract showed daily fluctuations with maximum staining intensity in the middle of the day and weakest staining at the end of the day. Quantification via enzyme-linked immunosorbent assay and quantitative liquid chromatography coupled to electrospray ionization tandem mass spectrometry, likewise, showed higher GABA levels in the optic lobe during the inactivity phase of the cockroach during the day and lower levels during its activity phase at dusk. Our data further support the hypothesis that light- and PDF-dependently the circadian clock network of the cockroach controls GABA levels and thereby promotes sleep during the day.eng
dcterms.accessRightsopen access
dcterms.creatorMassah, Azar
dcterms.creatorNeupert, Susanne
dcterms.creatorBrodesser, Susanne
dcterms.creatorHomberg, Uwe
dcterms.creatorStengl, Monika
dcterms.source.identifiereissn:1096-9861
dcterms.source.issueIssue 5
dcterms.source.journalJCN (The journal of comparative neurology)eng
dcterms.source.pageinfo770-791
dcterms.source.volumeVolume 530
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