Transcriptomic, peptidomic, and mass spectrometry imaging analysis of the brain in the ant Cataglyphis nodus

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dc.date.accessioned2021-08-23T12:11:16Z
dc.date.available2021-08-23T12:11:16Z
dc.date.issued2021-03-11
dc.description.sponsorshipGefördert im Rahmen des Projekts DEALger
dc.identifierdoi:10.17170/kobra-202107294433
dc.identifier.urihttp://hdl.handle.net/123456789/13151
dc.language.isoengeng
dc.relation.doidoi:10.1111/jnc.15346
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectCatalyhis nodus braineng
dc.subjectMALDI imagingeng
dc.subjectneuropeptideseng
dc.subjectneuropeptidomicseng
dc.subjectsocial insecteng
dc.subjecttranscriptomicseng
dc.subject.ddc570
dc.subject.swdCataglyphisger
dc.subject.swdGehirnger
dc.subject.swdMatrix-unterstützte Laser-Desorptionger
dc.subject.swdNeuropeptideger
dc.subject.swdSoziale Insektenger
dc.subject.swdGenger
dc.titleTranscriptomic, peptidomic, and mass spectrometry imaging analysis of the brain in the ant Cataglyphis noduseng
dc.typeAufsatz
dc.type.versionpublishedVersion
dcterms.abstractBehavioral flexibility is an important cornerstone for the ecological success of animals. Social Cataglyphis nodus ants with their age-related polyethism characterized by age-related behavioral phenotypes represent a prime example for behavioral flexibility. We propose neuropeptides as powerful candidates for the flexible modulation of age-related behavioral transitions in individual ants. As the neuropeptidome of C. nodus was unknown, we collected a comprehensive peptidomic data set obtained by transcriptome analysis of the ants’ central nervous system combined with brain extract analysis by Q-Exactive Orbitrap mass spectrometry (MS) and direct tissue profiling of different regions of the brain by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS. In total, we identified 71 peptides with likely bioactive function, encoded on 49 neuropeptide-, neuropeptide-like, and protein hormone prepropeptide genes, including a novel neuropeptide-like gene (fliktin). We next characterized the spatial distribution of a subset of peptides encoded on 16 precursor proteins with high resolution by MALDI MS imaging (MALDI MSI) on 14 µm brain sections. The accuracy of our MSI data were confirmed by matching the immunostaining patterns for tachykinins with MSI ion images from consecutive brain sections. Our data provide a solid framework for future research into spatially resolved qualitative and quantitative peptidomic changes associated with stage-specific behavioral transitions and the functional role of neuropeptides in Cataglyphis ants.eng
dcterms.accessRightsopen access
dcterms.creatorHabenstein, Jens
dcterms.creatorSchmitt, Franziska
dcterms.creatorLiessem, Sander
dcterms.creatorLy, Alice
dcterms.creatorTrede, Dennis
dcterms.creatorWegener, Christian
dcterms.creatorPredel, Reinhard
dcterms.creatorRössler, Wolfgang
dcterms.creatorNeupert, Susanne
dcterms.source.identifiereissn:1471-4159
dcterms.source.issueIssue 2
dcterms.source.journalJournal of Neurochemistryeng
dcterms.source.pageinfo391-412
dcterms.source.volumeVolume 158
kup.iskupfalse

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