Yeast gene KTI13 (alias DPH8) operates in the initiation step of diphthamide synthesis on elongation factor 2

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dc.date.accessioned2023-12-01T11:54:37Z
dc.date.available2023-12-01T11:54:37Z
dc.date.issued2023-08-08
dc.description.sponsorshipGefördert durch den Publikationsfonds der Universität Kassel
dc.identifierdoi:10.17170/kobra-202312019147
dc.identifier.urihttp://hdl.handle.net/123456789/15249
dc.language.isoeng
dc.relation.doidoi:10.15698/mic2023.09.804
dc.rightsNamensnennung 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectbudding yeasteng
dc.subjectEF2 diphthamide modificationeng
dc.subjectdiphtheria toxineng
dc.subjecttRNA modificationeng
dc.subjectelongatoreng
dc.subjecttRNase zymocineng
dc.subject.ddc540
dc.subject.ddc570
dc.subject.ddc610
dc.subject.swdHefeartige Pilzeger
dc.subject.swdToxinger
dc.subject.swdDiphtherietoxinger
dc.titleYeast gene KTI13 (alias DPH8) operates in the initiation step of diphthamide synthesis on elongation factor 2eng
dc.typeAufsatz
dc.type.versionpublishedVersion
dcterms.abstractIn yeast, Elongator-dependent tRNA modifications are regulated by the Kti11•Kti13 dimer and hijacked for cell killing by zymocin, a tRNase ribotoxin. Kti11 (alias Dph3) also controls modification of elongation factor 2 (EF2) with diphthamide, the target for lethal ADP-ribosylation by diphtheria toxin (DT). Diphthamide formation on EF2 involves four biosynthetic steps encoded by the DPH1-DPH7 network and an ill-defined KTI13 function. On further examining the latter gene in yeast, we found that kti13Δ null-mutants maintain unmodified EF2 able to escape ADP-ribosylation by DT and to survive EF2 inhibition by sordarin, a diphthamide-dependent antifungal. Consistently, mass spectrometry shows kti13Δ cells are blocked in proper formation of amino-carboxyl-propyl-EF2, the first diphthamide pathway intermediate. Thus, apart from their common function in tRNA modification, both Kti11/Dph3 and Kti13 share roles in the initiation step of EF2 modification. We suggest an alias KTI13/DPH8 nomenclature indicating dual-functionality analogous to KTI11/DPH3.eng
dcterms.accessRightsopen access
dcterms.creatorArend, Meike
dcterms.creatorÜtkür, Koray
dcterms.creatorHawer, Harmen
dcterms.creatorMayer, Klaus
dcterms.creatorRanjan, Namit
dcterms.creatorAdrian, Lorenz
dcterms.creatorBrinkmann, Ulrich
dcterms.creatorSchaffrath, Raffael
dcterms.source.identifiereissn:2311-2638
dcterms.source.issueIssue No. 9
dcterms.source.journalMicrobial Celleng
dcterms.source.pageinfo195 - 203
dcterms.source.volumeVolume 10
kup.iskupfalse

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