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Microsatellites from Fosterella christophii (Bromeliaceae) by de novo transcriptome sequencing on the Pacific Biosciences RS platform

Wöhrmann, Tina
Huettel, Bruno
Wagner, Natascha
Weising, Kurt

• Premise of the study: Microsatellite markers were developed in Fosterella christophii (Bromeliaceae) to investigate the genetic diversity and population structure within the F. micrantha group, comprising F. christophii, F. micrantha, and F. villosula. • Methods and Results: Full-length cDNAs were isolated from F. christophii and sequenced on a Pacific Biosciences RS platform. A total of 1590 high-quality consensus isoforms were assembled into 971 unigenes containing 421 perfect microsatellites. Thirty primer sets were designed, of which 13 revealed a high level of polymorphism in three populations of F. christophii, with four to nine alleles per locus. Each of these 13 loci cross-amplified in the closely related species F. micrantha and F. villosula, with one to six and one to 11 alleles per locus, respectively. • Conclusions: The new markers are promising tools to study the population genetics of F. christophii and to discover species boundaries within the F. micrantha group.

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Gefördert durch den Publikationsfonds der Universität Kassel 
@article{urn:nbn:de:hebis:34-2016020549834,
  author    ={Wöhrmann, Tina and Huettel, Bruno and Wagner, Natascha and Weising, Kurt},
  title    ={Microsatellites from Fosterella christophii (Bromeliaceae) by de novo transcriptome sequencing on the Pacific Biosciences RS platform},
  copyright  ={https://rightsstatements.org/page/InC/1.0/},
  language ={en},
  year   ={2016}
}