Fungi associated with fine roots of Fraxinus excelsior affected by ash dieback detected by next-generation sequencing

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dc.date.accessioned2024-10-31T09:18:16Z
dc.date.available2024-10-31T09:18:16Z
dc.date.issued2024-05-04
dc.description.sponsorshipGefördert im Rahmen des Projekts DEALger
dc.description.sponsorshipThe project receives funding via the Waldklimafonds (WKF) funded by the German Federal Ministry of Food and Agriculture (BMEL) and Federal Ministry for the Environment, Nature Conservation, Nuclear Safety and Consumer Protection (BMUV) administrated by the Agency for Renewable Resources (FNR) under grant agreement No. 2219WK22D4.eng
dc.identifierdoi:10.17170/kobra-2024103111039
dc.identifier.urihttp://hdl.handle.net/123456789/16129
dc.language.isoeng
dc.relation.doidoi:10.1007/s41348-024-00923-z
dc.relation.projectidgrant agreement No. 2219WK22D4
dc.rightsNamensnennung 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectdemonstration projecteng
dc.subjectEuropean ash diebackeng
dc.subjectFraxinus excelsioreng
dc.subjectfine rootseng
dc.subjectmycobiomeeng
dc.subjectnext-generation sequencingeng
dc.subjectOxford Nanopore MinIONeng
dc.subjectDeconaeng
dc.subjectMicroecoeng
dc.subjectPipeCraft2eng
dc.subject.ddc500
dc.subject.ddc570
dc.subject.swdEscheger
dc.subject.swdPilzeger
dc.subject.swdWurzelger
dc.subject.swdSequenzanalyse <Chemie>ger
dc.titleFungi associated with fine roots of Fraxinus excelsior affected by ash dieback detected by next-generation sequencingeng
dc.typeAufsatz
dc.type.versionpublishedVersion
dcterms.abstractThe decline of European ash by dieback caused by Hymenoscyphus fraxineus together with stem collar necroses and rots caused by various fungi has been investigated intensively during the last years. Nevertheless, hitherto nearly nothing is known about the species diversity of the fungal rhizobiome of ash trees. Here we investigated the fine roots of affected ash trees on 15 sampling sites in 6 federal countries of Germany. Fine-root samples have been treated in three different sample regimes each as root-adhering soil, unsterilized fine roots and sterilized fine roots. The samples of trees in sampling sites were pooled to get an overview of the species-richness in the area. The next-generation sequencing platform Oxford Nanopore MinION was used to sequence the entire ITS of pooled probes. Most abundant phyla in all samples were the Basidiomycota and Ascomycota. Species richness in sterilized roots was significantly different from unsterilized roots and root-adhering soil. Surprisingly most abundant genera in sterilized roots were the genera Mycena, Mycenella and Delicatula, all of them agaricoids with saprophytic lifestyle. Eleven genera of Glomeromycota have been detected in various abundances, whereas the detection of H. fraxineus was neglectable.eng
dcterms.accessRightsopen access
dcterms.creatorLysenko, Ludmila
dcterms.creatorGriem, Erik
dcterms.creatorWagener, Patrick
dcterms.creatorLanger, Ewald Johannes
dcterms.source.identifiereissn:1861-3837
dcterms.source.issueIssue 5
dcterms.source.journalJournal of Plant Diseases and Protectioneng
dcterms.source.pageinfo1373-1385
dcterms.source.volumeVolume 131

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