Phospholipid fatty acids in soil—drawbacks and future prospects

dc.date.accessioned2022-02-25T15:31:05Z
dc.date.available2022-02-25T15:31:05Z
dc.date.issued2021-12-21
dc.description.sponsorshipGefördert im Rahmen des Projekts DEALger
dc.identifierdoi:10.17170/kobra-202201045354
dc.identifier.urihttp://hdl.handle.net/123456789/13654
dc.language.isoengeng
dc.relation.doidoi:10.1007/s00374-021-01613-w
dc.rightsNamensnennung 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectfungieng
dc.subjectbacteriaeng
dc.subjectactinobacteriaeng
dc.subjectfirmicuteseng
dc.subjectPLFA assignationeng
dc.subject.ddc570
dc.subject.swdPilzeger
dc.subject.swdBakterienger
dc.subject.swdStrahlenpilzeger
dc.subject.swdFirmicutesger
dc.subject.swdFettsäurenger
dc.subject.swdBodenkundeger
dc.titlePhospholipid fatty acids in soil—drawbacks and future prospectseng
dc.typeAufsatz
dc.type.versionpublishedVersion
dcterms.abstractThe current opinion and position paper highlights (1) correct assignation of indicator phospholipid fatty acids (PLFA), (2) specificity and recycling of PLFA in microorganisms, and (3) complete extraction and detection of PLFA. The straight-chain PLFA 14:0, 15:0, 16:0, and 17:0 occur in all microorganisms, i.e., also in fungi and not only in bacteria. If the phylum Actinobacteria is excluded from the group of Gram-positive bacteria, all remaining bacteria belong to the bacterial phylum Firmicutes, which should be considered. The PLFA 16:1ω5 should be used as an indicator for the biomass of arbuscular mycorrhizal fungi (AMF) as there is no experimental evidence that they occur in marked amounts in Gram-negative bacteria. Fungal PLFA should embrace the AMF-specific 16:1ω5. In the presence of plants, ergosterol should be used instead of the PLFA 18:2ω6,9 and 18:1ω9 as fungal indicators for Mucoromycotina, Ascomycota, and Basidiomycota. The majority of indicator PLFA are not fully specific for a certain microbial group. This problem might be intensified by recycling processes during decomposition to an unknown extent. Soil handling and extraction conditions should be further optimized. The reliability and accuracy of gas chromatographic separation need to be regularly checked against unintentional variations. PLFA analysis will still be of interest over the next decades as an important independent control of DNA-based methods.eng
dcterms.accessRightsopen access
dcterms.creatorJörgensen, Rainer Georg
dcterms.source.identifiereissn:1432-0789
dcterms.source.issueIssue 1
dcterms.source.journalBiology and Fertility of Soilseng
dcterms.source.pageinfo1-6
dcterms.source.volumeVolume 58
kup.iskupfalse

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