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A Novel Real Time PCR Method for the Detection and Quantification of Didymella pinodella in Symptomatic and Asymptomatic Plant Hosts

dc.date.accessioned2022-02-22T13:05:34Z
dc.date.available2022-02-22T13:05:34Z
dc.date.issued2021-12-31
dc.identifierdoi:10.17170/kobra-202202225796
dc.identifier.urihttp://hdl.handle.net/123456789/13649
dc.description.sponsorshipGefördert durch den Publikationsfonds der Universität Kassel
dc.language.isoeng
dc.rightsNamensnennung 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectpeaeng
dc.subjectfoot and root roteng
dc.subjectDidymella pinodellaeng
dc.subjectPhoma medicaginis var. pinodellaeng
dc.subjectqPCReng
dc.subjectwheateng
dc.subjectasymptomatic infectionseng
dc.subject.ddc630
dc.titleA Novel Real Time PCR Method for the Detection and Quantification of Didymella pinodella in Symptomatic and Asymptomatic Plant Hostseng
dc.typeAufsatz
dcterms.abstractDidymella pinodella is the major pathogen of the pea root rot complex in Europe. This wide host range pathogen often asymptomatically colonizes its hosts, making the control strategies challenging. We developed a real-time PCR assay for the detection and quantification of D. pinodella based on the TEF-1 alpha gene sequence alignments. The assay was tested for specificity on a 54-isolate panel representing 35 fungal species and further validated in symptomatic and asymptomatic pea and wheat roots from greenhouse tests. The assay was highly consistent across separate qPCR reactions and had a quantification/detection limit of 3.1 pg of target DNA per reaction in plant tissue. Cross-reactions were observed with DNA extracts of five Didymella species. The risk of cross contamination, however, is low as the non-targets have not been associated with pea previously and they were amplified with at least 1000-fold lower sensitivity. Greenhouse inoculation tests revealed a high correlation between the pathogen DNA quantities in pea roots and pea root rot severity and biomass reduction. The assay also detected D. pinodella in asymptomatic wheat roots, which, despite the absence of visible root rot symptoms, caused wheat biomass reduction. This study provides new insights into the complex life style of D. pinodella and can assist in better understanding the pathogen survival and spread in the environment.eng
dcterms.accessRightsopen access
dcterms.creatorŠišić, Adnan
dcterms.creatorOberhänsli, Thomas
dcterms.creatorBaćanović-Šišić, Jelena
dcterms.creatorHohmann, Pierre
dcterms.creatorFinckh, Maria Renate
dc.relation.doidoi:10.3390/jof8010041
dc.subject.swdInfektionger
dc.subject.swdErkennungger
dc.subject.swdEchtzeitbildverarbeitungger
dc.subject.swdQuantifizierungger
dc.subject.swdErbseger
dc.subject.swdWeizenger
dc.subject.swdWurzelfäuleger
dc.type.versionpublishedVersion
dcterms.source.identifiereissn:2309-608X
dcterms.source.issueIssue 1
dcterms.source.journalJournal of Fungieng
dcterms.source.volumeVolume 8
kup.iskupfalse
dcterms.source.articlenumber41


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