Aufsatz
Artikel (Publikationen im Open Access gefördert durch die UB)
Development of 18 Polymorphic Microsatellite Markers for Vinca minor (Apocynaceae) via 454 Pyrosequencing
Zusammenfassung
• Premise of the study: Polymorphic microsatellite markers were developed in Vinca minor (Apocynaceae) to evaluate the level of clonality, population structure, and genetic diversity of the species within its native and introduced range.
• Methods and Results: A total of 1371 microsatellites were found in 43,565 reads from 454 pyrosequencing of genomic V. minor DNA. Additional microsatellite loci were mined from publicly available cDNA sequences. After several rounds of screening, 18 primer pairs flanking di-, tri-, or tetranucleotide repeats were identified that revealed high levels of genetic diversity in two native Italian populations, with two to 11 alleles per locus. Clonal growth predominated in two populations from the introduced range in Germany. Five loci successfully cross-amplified in three additional Vinca species.
• Conclusions: The novel polymorphic microsatellite markers are promising tools for studying clonality and population genetics of V. minor and for assessing the historical origin of Central European populations.
• Methods and Results: A total of 1371 microsatellites were found in 43,565 reads from 454 pyrosequencing of genomic V. minor DNA. Additional microsatellite loci were mined from publicly available cDNA sequences. After several rounds of screening, 18 primer pairs flanking di-, tri-, or tetranucleotide repeats were identified that revealed high levels of genetic diversity in two native Italian populations, with two to 11 alleles per locus. Clonal growth predominated in two populations from the introduced range in Germany. Five loci successfully cross-amplified in three additional Vinca species.
• Conclusions: The novel polymorphic microsatellite markers are promising tools for studying clonality and population genetics of V. minor and for assessing the historical origin of Central European populations.
Zitierform
In: Applications in plant sciences. - Washington, DC : BioOne, 2015, 3 (5), 1500015,1-4Förderhinweis
Gefördert durch den Publikationsfonds der Universität KasselSammlung(en)
Publikationen (Fachgebiet Botanik (Systematik und Morphologie))Artikel (Publikationen im Open Access gefördert durch die UB)
Zitieren
@article{urn:nbn:de:hebis:34-2015063048634,
author={Moeller, Sina and Wöhrmann, Tina and Huettel, Bruno and Weising, Kurt},
title={Development of 18 Polymorphic Microsatellite Markers for Vinca minor (Apocynaceae) via 454 Pyrosequencing},
year={2015}
}
0500 Oax 0501 Text $btxt$2rdacontent 0502 Computermedien $bc$2rdacarrier 1100 2015$n2015 1500 1/eng 2050 ##0##urn:nbn:de:hebis:34-2015063048634 3000 Moeller, Sina 3010 Wöhrmann, Tina 3010 Huettel, Bruno 3010 Weising, Kurt 4000 Development of 18 Polymorphic Microsatellite Markers for Vinca minor (Apocynaceae) via 454 Pyrosequencing / Moeller, Sina 4030 4060 Online-Ressource 4085 ##0##=u http://nbn-resolving.de/urn:nbn:de:hebis:34-2015063048634=x R 4204 \$dAufsatz 4170 7136 ##0##urn:nbn:de:hebis:34-2015063048634
2015-06-30T12:17:16Z 2015-06-30T12:17:16Z 2015 2168-0450 urn:nbn:de:hebis:34-2015063048634 http://hdl.handle.net/123456789/2015063048634 Gefördert durch den Publikationsfonds der Universität Kassel eng Urheberrechtlich geschützt https://rightsstatements.org/page/InC/1.0/ 570 Development of 18 Polymorphic Microsatellite Markers for Vinca minor (Apocynaceae) via 454 Pyrosequencing Aufsatz • Premise of the study: Polymorphic microsatellite markers were developed in Vinca minor (Apocynaceae) to evaluate the level of clonality, population structure, and genetic diversity of the species within its native and introduced range. • Methods and Results: A total of 1371 microsatellites were found in 43,565 reads from 454 pyrosequencing of genomic V. minor DNA. Additional microsatellite loci were mined from publicly available cDNA sequences. After several rounds of screening, 18 primer pairs flanking di-, tri-, or tetranucleotide repeats were identified that revealed high levels of genetic diversity in two native Italian populations, with two to 11 alleles per locus. Clonal growth predominated in two populations from the introduced range in Germany. Five loci successfully cross-amplified in three additional Vinca species. • Conclusions: The novel polymorphic microsatellite markers are promising tools for studying clonality and population genetics of V. minor and for assessing the historical origin of Central European populations. open access In: Applications in plant sciences. - Washington, DC : BioOne, 2015, 3 (5), 1500015,1-4 Moeller, Sina Wöhrmann, Tina Huettel, Bruno Weising, Kurt doi:10.3732/apps.1500015
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