Date
2022-07-22Author
Swenson, Stephanie J.Eichler, LisaHörren, ThomasKolter, AndreasKöthe, SebastianLehmann, Gerlind U. C.Meinel, GotthardMühlethaler, RolandSorg, MartinGemeinholzer, BirgitSubject
570 Life sciences; biology 580 Plants; biology 590 Animals; zoology DeutschlandFalleBiomonitoringInsektensterbenNaturschutzLandschaftsentwicklungMetadata
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The potential of metabarcoding plant components of Malaise trap samples to enhance knowledge of plant-insect interactions
Abstract
The worldwide rapid declines in insect and plant abundance and diversity that have occurred in the past decades have gained public attention and demand for political actions to counteract these declines are growing. Rapid large-scale biomonitoring can aid in observing these changes and provide information for decisions for land management and species protection. Malaise traps have long been used for insect sampling and when insects are captured in these traps, they carry traces of plants they have visited on the body surface or as digested food material in the gut contents. Metabarcoding offers a promising method for identifying these plant traces, providing insight into the plants with which insects are directly interacting at a given time. To test the efficacy of DNA metabarcoding with these sample types, 79 samples from 21 sites across Germany were analysed with the ITS2 barcode. This study, to our knowledge, is the first examination of metabarcoding plant DNA traces from Malaise trap samples. Here, we report on the feasibility of sequencing these sample types, analysis of the resulting taxa, the usage of cultivated plants by insects near nature conservancy areas and the detection of rare and neophyte species. Due to the frequency of contamination and false positive reads, isolation and PCR negative controls should be used in every reaction. Metabarcoding has advantages in efficiency and resolution over microscopic identification of pollen and is the only possible identification method for the other plant traces from Malaise traps and could provide a broad utility for future studies of plant-insect interactions.
Citation
In: Metabarcoding and Metagenomics (MBMG) Volume 6 (2022-07-22) , S. 227-238 ; eissn:2534-9708Sponsorship
Gefördert durch den Publikationsfonds der Universität KasselCitation
@article{doi:10.17170/kobra-202301207417,
author={Swenson, Stephanie J. and Eichler, Lisa and Hörren, Thomas and Kolter, Andreas and Köthe, Sebastian and Lehmann, Gerlind U. C. and Meinel, Gotthard and Mühlethaler, Roland and Sorg, Martin and Gemeinholzer, Birgit},
title={The potential of metabarcoding plant components of Malaise trap samples to enhance knowledge of plant-insect interactions},
journal={Metabarcoding and Metagenomics (MBMG)},
year={2022}
}
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2023-01-20T16:00:26Z 2023-01-20T16:00:26Z 2022-07-22 doi:10.17170/kobra-202301207417 http://hdl.handle.net/123456789/14380 Gefördert durch den Publikationsfonds der Universität Kassel eng Namensnennung 4.0 International http://creativecommons.org/licenses/by/4.0/ biomonitoring DNA metabarcoding insect decline landscape change nature conservation plant-insect interactions 570 580 590 The potential of metabarcoding plant components of Malaise trap samples to enhance knowledge of plant-insect interactions Aufsatz The worldwide rapid declines in insect and plant abundance and diversity that have occurred in the past decades have gained public attention and demand for political actions to counteract these declines are growing. Rapid large-scale biomonitoring can aid in observing these changes and provide information for decisions for land management and species protection. Malaise traps have long been used for insect sampling and when insects are captured in these traps, they carry traces of plants they have visited on the body surface or as digested food material in the gut contents. Metabarcoding offers a promising method for identifying these plant traces, providing insight into the plants with which insects are directly interacting at a given time. To test the efficacy of DNA metabarcoding with these sample types, 79 samples from 21 sites across Germany were analysed with the ITS2 barcode. This study, to our knowledge, is the first examination of metabarcoding plant DNA traces from Malaise trap samples. Here, we report on the feasibility of sequencing these sample types, analysis of the resulting taxa, the usage of cultivated plants by insects near nature conservancy areas and the detection of rare and neophyte species. Due to the frequency of contamination and false positive reads, isolation and PCR negative controls should be used in every reaction. Metabarcoding has advantages in efficiency and resolution over microscopic identification of pollen and is the only possible identification method for the other plant traces from Malaise traps and could provide a broad utility for future studies of plant-insect interactions. open access Swenson, Stephanie J. Eichler, Lisa Hörren, Thomas Kolter, Andreas Köthe, Sebastian Lehmann, Gerlind U. C. Meinel, Gotthard Mühlethaler, Roland Sorg, Martin Gemeinholzer, Birgit doi:10.3897/mbmg.6.85213 Deutschland Falle Biomonitoring Insektensterben Naturschutz Landschaftsentwicklung publishedVersion eissn:2534-9708 Metabarcoding and Metagenomics (MBMG) 227-238 Volume 6 false 85213
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