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Date
2024-03-19Author
Jörgensen, Rainer GeorgHemkemeyer, MichaelBeule, LukasIskakova, JanylOskonbaeva, ZhyldyzRummel, Pauline SophieSchwalb, Sanja AnnabellWichern, FlorianSubject
500 Science 570 Life sciences; biology BiomasseKohlenstoffBodenmikrobiologieBakterienArchaebakterienPilzeErgosterinMetadata
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Aufsatz
A hitchhiker’s guide: estimates of microbial biomass and microbial gene abundance in soil
Abstract
Information on microbial biomass carbon (MBC) is crucial to assess their stocks and role for plant nutrient release in soil. Next to fumigation-extraction, molecular methods are routinely used to estimate the contribution of fungi, bacteria, and archaea to the soil microbial community. However, more information on the links between these different indices would deepen the understanding of microbial processes. The current study is based on 11 datasets, which contain MBC and MBN data obtained by fumigation-extraction and information on bacterial, archaeal, and fungal gene abundance, totalling 765 data points from agricultural, forest, and rangeland soils. Some of these datasets additionally provide information on double-stranded deoxyribonucleic acid (dsDNA) and fungal ergosterol. MBC varied around the median of 206 µg g−¹ soil. MBN followed with a median MB-C/N ratio of 4.1. Median microbial gene abundance declined from bacteria (96 × 10⁸) to archaea (4.4 × 10⁸) to fungi (1.8 × 10⁸). The median ratio of MBC/dsDNA was 15.8 and that of bacteria/dsDNA was 5.8 × 10⁸ µg−¹. The relationships between MBC and dsDNA as well as between bacterial gene abundance and dsDNA were both negatively affected by soil pH and positively by clay content. The median ergosterol/MBC and fungi/ergosterol ratios were 0.20% and 4.7 (n × 10⁸ µg−¹), respectively. The relationship between fungal gene abundance and ergosterol was negatively affected by soil pH and clay content. Our study suggests that combining fumigation-extraction with molecular tools allows more precise insights on the physiological interactions of soil microorganisms with their surrounding environment.
Citation
In: Biology and Fertility of Soils Volume 60 / Issue 4 (2024-03-19) , S. 457-470 ; eissn:1432-0789Sponsorship
Gefördert im Rahmen des Projekts DEALCitation
@article{doi:10.17170/kobra-2024042310060,
author={Jörgensen, Rainer Georg and Hemkemeyer, Michael and Beule, Lukas and Iskakova, Janyl and Oskonbaeva, Zhyldyz and Rummel, Pauline Sophie and Schwalb, Sanja Annabell and Wichern, Florian},
title={A hitchhiker’s guide: estimates of microbial biomass and microbial gene abundance in soil},
journal={Biology and Fertility of Soils},
year={2024}
}
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2024-05-17T11:18:36Z 2024-05-17T11:18:36Z 2024-03-19 doi:10.17170/kobra-2024042310060 http://hdl.handle.net/123456789/15754 Gefördert im Rahmen des Projekts DEAL eng Namensnennung 4.0 International http://creativecommons.org/licenses/by/4.0/ MBC dsDNA bacteria archaea fungi ergosterol 500 570 A hitchhiker’s guide: estimates of microbial biomass and microbial gene abundance in soil Aufsatz Information on microbial biomass carbon (MBC) is crucial to assess their stocks and role for plant nutrient release in soil. Next to fumigation-extraction, molecular methods are routinely used to estimate the contribution of fungi, bacteria, and archaea to the soil microbial community. However, more information on the links between these different indices would deepen the understanding of microbial processes. The current study is based on 11 datasets, which contain MBC and MBN data obtained by fumigation-extraction and information on bacterial, archaeal, and fungal gene abundance, totalling 765 data points from agricultural, forest, and rangeland soils. Some of these datasets additionally provide information on double-stranded deoxyribonucleic acid (dsDNA) and fungal ergosterol. MBC varied around the median of 206 µg g−¹ soil. MBN followed with a median MB-C/N ratio of 4.1. Median microbial gene abundance declined from bacteria (96 × 10⁸) to archaea (4.4 × 10⁸) to fungi (1.8 × 10⁸). The median ratio of MBC/dsDNA was 15.8 and that of bacteria/dsDNA was 5.8 × 10⁸ µg−¹. The relationships between MBC and dsDNA as well as between bacterial gene abundance and dsDNA were both negatively affected by soil pH and positively by clay content. The median ergosterol/MBC and fungi/ergosterol ratios were 0.20% and 4.7 (n × 10⁸ µg−¹), respectively. The relationship between fungal gene abundance and ergosterol was negatively affected by soil pH and clay content. Our study suggests that combining fumigation-extraction with molecular tools allows more precise insights on the physiological interactions of soil microorganisms with their surrounding environment. open access Jörgensen, Rainer Georg Hemkemeyer, Michael Beule, Lukas Iskakova, Janyl Oskonbaeva, Zhyldyz Rummel, Pauline Sophie Schwalb, Sanja Annabell Wichern, Florian doi:10.1007/s00374-024-01810-3 Biomasse Kohlenstoff Bodenmikrobiologie Bakterien Archaebakterien Pilze Ergosterin publishedVersion eissn:1432-0789 Issue 4 Biology and Fertility of Soils 457-470 Volume 60 false
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