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Effects of Mangosteen Peel Phenolic Compounds on Tilapia Skin Collagen-Based Mineralized Scaffold Properties
(2022-09-16)
A proper valorization of biological waste sources for an effective conversion into composites for tissue engineering is discussed in this study. Hence, the collagen and the phenolic compound applied in this investigation were extracted from waste sources, respectively, fish industry rejects and the peels of the mangosteen fruit. Porous scaffolds were prepared by combining both components at different compositions and mineralized at different temperatures to evaluate the modifications in the biomimetic formation of ...
Aufsatz
Spatial and Temporal Variations of Stable Isotopes in Precipitation in the Mountainous Region, North Hesse
(2022-12-01)
Patterns of stable isotopes of water (¹⁸O and ²H) in precipitation have been used as tracers for analyzing environmental processes which can be changed by factors such as the topography or meteorological variables. In this study, we investigated the isotopic data in precipitation for one year in the low mountain range of North Hesse, Germany, and analyzed mainly for altitude, rainfall amount, and air temperature effects on a regional scale. The results indicate that the isotopic composition expressed an altitude ...
Aufsatz
Yeast gene KTI13 (alias DPH8) operates in the initiation step of diphthamide synthesis on elongation factor 2
(2023-08-08)
In yeast, Elongator-dependent tRNA modifications are regulated by the Kti11•Kti13 dimer and hijacked for cell killing by zymocin, a tRNase ribotoxin. Kti11 (alias Dph3) also controls modification of elongation factor 2 (EF2) with diphthamide, the target for lethal ADP-ribosylation by diphtheria toxin (DT). Diphthamide formation on EF2 involves four biosynthetic steps encoded by the DPH1-DPH7 network and an ill-defined KTI13 function. On further examining the latter gene in yeast, we found that kti13Δ null-mutants ...
Aufsatz
Combined leaching and plant uptake simulations of PFOA and PFOS under field conditions
(2020-08-31)
Per- and polyfluoroalkyl substances (PFASs) are used in industrial production and manufacturing but were repeatedly detected in agricultural soils and therefore in cash crops in recent years. Dissipation of perfluoroalkyl acids (PFAAs), a sub-group of PFASs, in the environment was rather attributed to the formation of non-extractable residues (NER) than to degradation or transformation. Currently, there are no models describing the fate of PFAAs in the soil-plant continuum under field conditions, which hampers an ...
Aufsatz
Importance of sources of variability, scales and experimental design: A case study about the effects of biochar and slurry application on soil properties in agricultural silty loam soils
(2021-04-17)
In designed experiments, different sources of variability and an adequate scale of measurement need to be considered, but not all approaches in common usage are equally valid. In order to elucidate the importance of sources of variability and choice of scale, we conducted an experiment where the effects of biochar and slurry applications on soil properties related to soil fertility were studied for different designs: (a) for a field-scale sampling design with either a model soil (without natural variability) as an ...
Aufsatz
Application of mixed-effects modelling and rule-based models to explain copper variation in soil profiles of southern Germany
(2022-05-25)
Copper (Cu) is an essential element for plants and microorganisms and at larger concentrations a toxic pollutant. A number of factors controlling Cu dynamics have been reported, but information on quantitative relationships is scarce. We aimed to (i) quantitatively describe and predict soil Cu concentrations (CuAR) in aqua regia considering site-specific effects and effects of pH, soil organic carbon (SOC) and cation exchange capacity (CEC), and (ii) study the suitability of mixed-effects modelling and rule-based ...
Aufsatz
Binding of the Human 14-3-3 Isoforms to Distinct Sites in the Leucine-Rich Repeat Kinase 2
(2020-04-07)
Proteins of the 14-3-3 family are well known modulators of the leucine-rich repeat kinase 2 (LRRK2) regulating kinase activity, cellular localization, and ubiquitylation. Although binding between those proteins has been investigated, a comparative study of all human 14-3-3 isoforms interacting with LRRK2 is lacking so far. In a comprehensive approach, we quantitatively analyzed the interaction between the seven human 14-3-3 isoforms and LRRK2-derived peptides covering both, reported and putative 14-3-3 binding sites. ...
Aufsatz
Mineralization of Phosphorylated Fish Skin Collagen/Mangosteen Scaffolds as Potential Materials for Bone Tissue Regeneration
(2021-05-13)
In this study, a potential hard tissue substitute was mimicked using collagen/mangosteen porous scaffolds. Collagen was extracted from Tilapia fish skin and mangosteen from the waste peel of the respective fruit. Sodium trimetaphosphate was used for the phosphorylation of these scaffolds to improve the nucleation sites for the mineralization process. Phosphate groups were incorporated in the collagen structure as confirmed by their attenuated total reflection Fourier transform infrared (ATR-FTIR) bands. The phosphorylation ...
Aufsatz
Prediction of total carotenoids, color, and moisture content of carrot slices during hot air drying using non-invasive hyperspectral imaging technique
(2022-02-04)
The objective of this paper was to evaluate the performance of Partial Least Square Regression (PLSR) model and to assess the statistical agreement between two different measurement techniques, that is, Vis–NIR hyperspectral imaging (HSI) and standard laboratory methods for quality evaluation of dried carrots throughout the hot-air drying process. Carrots at commercial maturity of 3.5 months after planting were harvested in two seasons (2017 and 2018) and dried in a convective hot air dryer at 50°C, 60°C, and 70°C. ...
Aufsatz
RedOx regulation of LRRK2 kinase activity by active site cysteines
(2024-04-03)
Mutations of the human leucine-rich repeat kinase 2 (LRRK2) have been associated with both, idiopathic and familial Parkinson’s disease (PD). Most of these pathogenic mutations are located in the kinase domain (KD) or GTPase domain of LRRK2. In this study we describe a mechanism in which protein kinase activity can be modulated by reversible oxidation or reduction, involving a unique pair of adjacent cysteines, the “CC” motif. Among all human protein kinases, only LRRK2 contains this “CC” motif (C2024 and C2025) in ...