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dc.description.sponsorshipGefördert durch den Publikationsfonds der Universität Kassel
dc.rightsUrheberrechtlich geschützt
dc.titleMicrosatellites from Fosterella christophii (Bromeliaceae) by de novo transcriptome sequencing on the Pacific Biosciences RS platformeng
dcterms.abstract• Premise of the study: Microsatellite markers were developed in Fosterella christophii (Bromeliaceae) to investigate the genetic diversity and population structure within the F. micrantha group, comprising F. christophii, F. micrantha, and F. villosula. • Methods and Results: Full-length cDNAs were isolated from F. christophii and sequenced on a Pacific Biosciences RS platform. A total of 1590 high-quality consensus isoforms were assembled into 971 unigenes containing 421 perfect microsatellites. Thirty primer sets were designed, of which 13 revealed a high level of polymorphism in three populations of F. christophii, with four to nine alleles per locus. Each of these 13 loci cross-amplified in the closely related species F. micrantha and F. villosula, with one to six and one to 11 alleles per locus, respectively. • Conclusions: The new markers are promising tools to study the population genetics of F. christophii and to discover species boundaries within the F. micrantha group.eng
dcterms.accessRightsopen access
dcterms.bibliographicCitationIn: Applications in plant sciences. - Washington, DC : BioOne, 2016, 4 (1):1500084, 1-4
dcterms.creatorWöhrmann, Tina
dcterms.creatorHuettel, Bruno
dcterms.creatorWagner, Natascha
dcterms.creatorWeising, Kurt

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