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Dissertation
Investigation of the functional synergy and involvement of different tRNA modifications in translational control
(2020-07-02)
Transfer RNAs are essential adapter molecules employed in the translational process and are highly modified all over their structure. Specific modification of the four canonical ribonucleotides (A, U, C, G) is accomplished by various proteins and/or protein complexes, ranging from simple isomerisations (e.g. Ψ) and methylations (e.g. m5C) up to complex chemical structures (e.g. mcm5s2U, ct6A). The functions of tRNA modifications are linked to the specific position and may influence translation by supporting the native ...
Dissertation
Association of neighboring β-Strands to form the β-barrel structure of the voltage-dependent anion channel, human isoform 1 (hVDAC1) precedes membrane insertion and is largely driven by polar interactions between basic and acidic amino acid side-chains
(2020)
To date, all biophysical folding studies on β-barrel membrane proteins from outer membranes (OMPs) indicated a coupled mechanism of folding and membrane insertion. Most of these studies were performed with smaller OMPs from bacteria. Here we have investigated folding and insertion of a mitochondrial OMP, the voltage-dependent anion-selective channel (VDAC), human isoform 1 (hVDAC1). To examine whether folding and insertion are coupled for hVDAC1, the association of neighboring β-strands of the 19-stranded β-barrel ...
Dissertation
Geospatial crop yield modeling for climate change risk transfer, impact assessment, and adaptation strategies
(2023)
Changing climate and weather patterns increase the pressure on food production systems worldwide, significantly impacting livelihoods and economies at various scales. Understanding and adapting/mitigating climate change are essential to cope with the adverse effects of climate change. Biophysical crop modeling offers a unique way to assess the pros and cons of different adaptation strategies because they integrate physical and biological principles to simulate the use and allocation of captured resources mechanistically. ...
Dissertation
Market-driven harvesting of non-timber forest products and the governance of communal forests in the south-east lowveld of Zimbabwe.
(2023-07)
Despite forests providing a wide range of important products and services for much of Zimbabwe’s population, natural forests are threatened by deforestation. The highest rates of deforestation occur in communal forests. Communal forest resources in Zimbabwe show evidence of increased degradation and signs of a breakdown of local institutions for resources management. These signs of a breakdown of local institutions are coupled with evidence of a lack of any emerging alternative institutions for conservation of forest ...
Dissertation
Regulatory mechanisms of Protein kinase A - Isoform mediated fine-tuning
(2023)
cAMP-dependent protein kinase (PKA) is a crucial part in eukaryotic signal transduction and controls biological processes. With a multitude of substrates, a great variety of cellular functions are regulated by PKA. Regulation of metabolism, cell growth and -differentiation, conductivity of ion channels, movement of sperm cells, embryonic development, neuroplasticity as well as conversion of short- to long-term memory are just examples for the many tasks of the kinase. To achieve and coordinate these diverse functions, ...
Dissertation
Lignocellulosic biorefinery with a thermo-biological pretreatment concept
(2023)
There is great interest, not only in technologically advanced societies, in developing innovative sources of raw materials, especially lignocellulosic biomasses such as cereal straw, which do not interfere with food production. In Germany, 4 – 9 million tons of cereal straw, mainly wheat, are utilized annually, corresponding to an energy potential of 57 – 129 PJ/a. Lignocellulose, found in plant cell walls, consists of hemicellulose, cellulose, and lignin, and it requires complex fractionation to enable its polymers ...
Dissertation
Pathogenic Mutations in Catalytic Subunit Isoforms of the cAMP-Dependent Protein Kinase
(2023)
Protein kinase A (PKA) is an integral part in a myriad of cellular pathways and a main effector of the second messenger cAMP. Since its discovery in the late 1960s, PKA has become one of the best-studied eukaryotic protein kinases in terms of enzymatic and structural features. Pathological implications of PKA have just started to emerge. There are three main isoforms (α, β, γ) of the catalytic subunit of PKA (PKA-C) which constitute a holoenzyme (R2C2) by association with a regulatory subunit (PKA-R) dimer. While ...
Dissertation
Biochemical Characterization of Microtubule-Associated Serine/Threonine Kinases
(2023)
The association of human microtubule-associated serine/threonine (MAST) kinases with different diseases, such as neurodevelopmental disorders and cancer, leads to hight interest in understanding the function of these understudied kinases. This PhD thesis provides the first consolidated biochemical analysis of three different human MAST kinases (MAST1-3) and the Drosophila melanogaster homologue drop out protein (DOP).
Dissertation
Functional Analysis of Src42A in embryonic epithelial morphogenesis of Drosophila melanogaster
(2023)
Src42A is one of the Src family kinases (SFKs) present in Drosophila melanogaster. In mammals there are 8 SFKs and so far, a loss of function analysis of SFKs were difficult to conduct due to their functional redundancy. In Drosophila, there are only two SFKs namely Src42A and Src64B, the former is essential for embryonic development and the latter is a non-essential gene. Since Src42A is supplied as a maternal component, the zygotic mutant were showing defects in later developmental stages, because the contribution ...