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Aufsatz
SSPIM: a beam shaping toolbox for structured selective plane illumination microscopy
(2018-07-03)
Selective plane illumination microscopy (SPIM) represents a preferred method in dynamic tissue imaging, because it combines high spatiotemporal resolution with low phototoxicity. The OpenSPIM system was developed to provide an accessible and flexible microscope set-up for non-specialist users. Here, we report Structured SPIM (SSPIM), which offers an open-source, user-friendly and compact toolbox for beam shaping to be applied within the OpenSPIM platform. SSPIM is able to generate digital patterns for a wide range ...
Aufsatz
Short-spored Subulicystidium (Trechisporales, Basidiomycota): high morphological diversity and only partly clear species boundaries
(2018-06-27)
Diversity of corticioid fungi (resupinate Basidiomycota), especially outside the northern temperate climatic zone, remains poorly explored. Furthermore, most of the known species are delimited by morphological concepts only and, not rarely, these concepts are too broad and need to be tested by molecular tools. For many decades, the delimitation of species in the genus Subulicystidium (Hydnodontaceae, Trechisporales) was a challenge for mycologists. The presence of numerous transitional forms as to basidiospore size ...
Aufsatz
S-Adenosyl-L-Homocysteine Hydrolase Inhibition by a Synthetic Nicotinamide Cofactor Biomimetic
(2018-03-21)
S-adenosyl-L-homocysteine (SAH) hydrolases (SAHases) are involved in the regulation of methylation reactions in many organisms and are thus crucial for numerous cellular functions. Consequently, their dysregulation is associated with severe health problems. The SAHase-catalyzed reaction is reversible and both directions depend on the redox activity of nicotinamide adenine dinucleotide (NAD+) as a cofactor. Therefore, nicotinamide cofactor biomimetics (NCB) are a promising tool to modulate SAHase activity. In the ...
Aufsatz
tRNA anticodon loop modifications ensure protein homeostasis and cell morphogenesis in yeast
(2016-08-05)
Using budding yeast, we investigated a negative interaction network among genes for tRNA modifications previously implicated in anticodon-codon interaction: 5-methoxy-carbonyl-methyl-2-thio-uridine (mcm5s2U34: ELP3, URM1), pseudouridine (Ψ38/39: DEG1) and cyclic N6-threonyl-carbamoyl-adenosine (ct6A37: TCD1). In line with functional cross talk between these modifications, we find that combined removal of either ct6A37 or Ψ38/39 and mcm5U34 or s2U34 results in morphologically altered cells with synthetic growth defects. ...
Konferenzveröffentlichung
Das Potential von Virtual Reality für das Lernen von Chemie an Schulen und Hochschulen
(2019-09)
Das Potential von Virtual Reality für das Lehren und Lernen von Chemie an Schulen und Hochschulen wird dargelegt und diskutiert.
Dissertation
The tRNA binding protein Kti12 and its role in the regulation of Elongator complex
(2020-06-08)
[Summary] Kti12 is a conserved eukaryotic protein showing sequence homology to O-phosphoseryltRNA kinase (Pstk), which is required for the synthesis of selenocysteine tRNA. Saccharomyces cerevisiae Kti12 was originally discovered in a genetic screening for resistance against zymocin, a protein toxin produced by Kluyveromyces lactis. The cytotoxicity of zymocin lies in its anticodon nuclease activity, causing tRNA depletion and cell death in sensitive yeast cells. It recognizes and cleaves a particular wobble uridine ...
Dissertation
Lösungen linearer Polynomgleichungen in Funktionenkörpern und Uniformisierbarkeit von t-Moduln
(2018)
Bei abelschen t-Moduln über Funktionenkörpern, denen der Ring F_q[t] zugrunde liegt, spielt die Frage der Uniformisierbarkeit eine wichtige Rolle. In dieser Arbeit werden t-Moduln betrachtet, die durch
t = τ^2 + A τ+ θ
gegeben sind, wobei τ den q-Frobenius-Endomorphismus bezeichnet, A eine (d x d)-Matrix mit d = 2 ist und θ eine Unbestimmte über F_q ist, die als Skalar (der t entspricht) im Funktionenkörper F_q(( 1/θ )) aufgefasst wird.
Nach einem Satz von Anderson aus der grundlegenden Arbeit “t-motives” (1986) ...
Aufsatz
Requirement of the Dynein-Adaptor Spindly for Mitotic and Post-Mitotic Functions in Drosophila
(2018-03-30)
Spindly was originally identified as a specific regulator of Dynein activity at the kinetochore. In early prometaphase, Spindly recruits the Dynein/Dynactin complex, promoting the establishment of stable kinetochore-microtubule interactions and progression into anaphase. While details of Spindly function in mitosis have been worked out in cultured human cells and in the C. elegans zygote, the function of Spindly within the context of an organism has not yet been addressed. Here, we present loss- and gain-of-function ...
Aufsatz
Protein Phosphatase Sit4 Affects Lipid Droplet Synthesis and Soraphen A Resistance Independent of Its Role in Regulating Elongator Dependent tRNA Modification
(2018-07-11)
The protein phosphatase Sit4 has been shown to be required for lipogenesis and resistance against the acetyl-CoA carboxylase inhibitor soraphen A. Since Sit4 is also required for biosynthesis of Elongator dependent tRNA modifications such as 5-methoxycarbonylmethyluridine (mcm5U), we investigated the relevance of tRNA modifications in lipogenesis and soraphen A response. While sit4 and Elongator (elp3) mutants copy defects in mcm5U formation and stress sensitivity, they do not share soraphen A sensitivity and low ...
Aufsatz
Extreme Ultraviolet to Visible Dispersed Single Photon Detection for Highly Sensitive Sensing of Fundamental Processes in Diverse Samples
(2018-05-23)
The detection of a single photon is the most sensitive method for sensing of photon emission. A common technique for single photon detection uses microchannel plate arrays combined with photocathodes and position sensitive anodes. Here, we report on the combination of such detectors with grating diffraction spectrometers, constituting a low-noise wavelength resolving photon spectroscopy apparatus with versatile applicability. We recapitulate the operation principle of such detectors and present the details of the ...