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SSPIM: a beam shaping toolbox for structured selective plane illumination microscopy
(2018-07-03)
Selective plane illumination microscopy (SPIM) represents a preferred method in dynamic tissue imaging, because it combines high spatiotemporal resolution with low phototoxicity. The OpenSPIM system was developed to provide an accessible and flexible microscope set-up for non-specialist users. Here, we report Structured SPIM (SSPIM), which offers an open-source, user-friendly and compact toolbox for beam shaping to be applied within the OpenSPIM platform. SSPIM is able to generate digital patterns for a wide range ...
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High-Purity Triggered Single-Photon Emission from Symmetric Single InAs/InP Quantum Dots around the Telecom C-Band Window
(2019-11-07)
The authors demonstrate pure triggered single‐photon emission from quantum dots (QDs) around the telecommunication C‐band window, with characteristics preserved under non‐resonant excitation at saturation, that is, the highest possible, lifetime‐limited emission rates. The direct measurement of emission dynamics reveals photoluminescence decay times in the range of (1.7–1.8) ns corresponding to maximal photon generation rates exceeding 0.5 GHz. The measurements of the second‐order correlation function exhibit, for ...
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Short-spored Subulicystidium (Trechisporales, Basidiomycota): high morphological diversity and only partly clear species boundaries
(2018-06-27)
Diversity of corticioid fungi (resupinate Basidiomycota), especially outside the northern temperate climatic zone, remains poorly explored. Furthermore, most of the known species are delimited by morphological concepts only and, not rarely, these concepts are too broad and need to be tested by molecular tools. For many decades, the delimitation of species in the genus Subulicystidium (Hydnodontaceae, Trechisporales) was a challenge for mycologists. The presence of numerous transitional forms as to basidiospore size ...
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S-Adenosyl-L-Homocysteine Hydrolase Inhibition by a Synthetic Nicotinamide Cofactor Biomimetic
(2018-03-21)
S-adenosyl-L-homocysteine (SAH) hydrolases (SAHases) are involved in the regulation of methylation reactions in many organisms and are thus crucial for numerous cellular functions. Consequently, their dysregulation is associated with severe health problems. The SAHase-catalyzed reaction is reversible and both directions depend on the redox activity of nicotinamide adenine dinucleotide (NAD+) as a cofactor. Therefore, nicotinamide cofactor biomimetics (NCB) are a promising tool to modulate SAHase activity. In the ...
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How to Improve Performance in Bayesian Inference Tasks: A Comparison of Five Visualizations
(2019-02-20)
Bayes’ formula is a fundamental statistical method for inference judgments in uncertain situations used by both laymen and professionals. However, since people often fail in situations where Bayes’ formula can be applied, how to improve their performance in Bayesian situations is a crucial question. We based our research on a widely accepted beneficial strategy in Bayesian situations, representing the statistical information in the form of natural frequencies. In addition to this numerical format, we used five ...
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Spatial and Temporal Dynamics of Urban Wetlands in an Indian Megacity over the Past 50 Years
(2020-02-17)
Asian megacities have attracted much scientific attention in the context of global urbanization, but few quantitative studies analyze wetland transformation in the rural–urban interface. With its rampant growth and transformation from a tree-lined “Garden City” to a busy megalopolis with often-blocked highways and large built-up areas, Bengaluru (Karnataka, S-India) is a good example for assessing how urbanization has led to the acute degradation of wetlands. We therefore investigated long-term land cover and wetland ...
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Construction and Use of a Simple Index of Urbanisation in the Rural–Urban Interface of Bangalore, India
(2017-11-21)
Urbanisation is a global trend rapidly transforming the biophysical and socioeconomic structures of metropolitan areas. To better understand (and perhaps control) these processes, more interdisciplinary research must be dedicated to the rural–urban interface. This also calls for a common reference system describing intermediate stages along a rural–urban gradient. The present paper constructs a simple index of urbanisation for villages in the Greater Bangalore Area, using GIS analysis of satellite images, and combining ...
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Cross-π-conjugated enediyne with multioptic metal binding sites
(2020-10-20)
The synthesis of an enediyne molecule functionlized with different metal coordination sites in a cross-pconjugated fashion is reported. Using Pd-mediated cross-coupling reactions, 2,20-bipyridine units were attached at the periphery of diazafluorenemethylidene to obtain a multitopic ligand. UV-vis spectrosopic investigations along with electrochemical analyses reveal electronic communication along the conjugated path reflected in red-shifted absorption spectra and shifts of reduction potentials. The properties of the ...
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Electrostatic Body Forces and their Implications for Cracks
(2023-03-24)
A Griffith-type crack in a dielectric solid under electromechanical loading is investigated by numerically solving the combined boundary value problem. The focus is on the contribution of electrostatically induced body forces, coming along with the still controversially discussed Maxwell stress tensor, to the crack tip loading. A 1/r-singularity of compressive stress is found to evolve at the crack tip, however, just being dominant over the mechanically induced 1/√r-behavior within a very small region
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Water Quality in Surface Water: A Preliminary Assessment of Heavy Metal Contamination of the Mashavera River, Georgia
(2018-03-23)
Water quality contamination by heavy metal pollution has severe effects on public health. In the Mashavera River Basin, an important agricultural area for the national food system in Georgia (e.g., vegetable, dairy and wine production), water contamination has multiple influences on the regional and country-wide health. With new industrial activities in the region, sediment extraction, and discharge of untreated wastewater into the river, its tributaries and irrigation canals, a comprehensive study of water quality ...