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Lipidome remodeling in response to nutrient replenishment requires the tRNA modifier Deg1/Pus3 in yeast
(2023-10-21)
In the yeast Saccharomyces cerevisiae, the absence of the pseudouridine synthase Pus3/Deg1, which modifies tRNA positions 38 and 39, results in increased lipid droplet (LD) content and translational defects. In addition, starvation-like transcriptome alterations and induced protein aggregation were observed. In this study, we show that the deg1 mutant increases specific misreading errors. This could lead to altered expression of the main regulators of neutral lipid synthesis which are the acetyl-CoA carboxylase (Acc1), ...
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Anthropogenic and natural disturbances increase local genetic diversity in an early spring geophyte (Ficaria verna Huds)
(2023-12-26)
The tetraploid Ficaria verna is a common spring geophyte in central Europe and is considered invasive in the USA and Canada. It is considered an almost seed-sterile taxon, relying on vegetative reproduction by underground tubers and aerial bulbils. Recent studies have revealed high levels of population genetic diversity in F. verna, raising the question of how genetic diversity is maintained and which factors may be responsible for the observed patterns. Polymorphic nuclear microsatellite markers were established to ...
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Galactosamine and mannosamine are integral parts of bacterial and fungal extracellular polymeric substances
(2024-03-22)
Extracellular polymeric substances (EPS) are produced by microorganisms and interact to form a complex matrix called biofilm. In soils, EPS are important contributors to the microbial necromass and, thus, to soil organic carbon (SOC). Amino sugars (AS) are used as indicators for microbial necromass in soil, although the origin of galactosamine and mannosamine is largely unknown. However, indications exist that they are part of EPS. In this study, two bacteria and two fungi were grown in starch medium either with or ...
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Optimization of the bicinchoninic acid assay for quantifying carbohydrates of soil extracellular polymeric substances
(2023-12-23)
Background and aims
The bicinchoninic acid (BCA) method was not yet applied on soil extracts of extracellular polymeric substances (EPS) to quantify polysaccharides, although this might be possible by introducing a cleavage step to produce monosaccharides. A pre-extraction with CaCl₂ to remove interfering substances is usually performed before extracting EPS. The main objective of this study was to optimize the BCA assay for total carbohydrates quantification by applying a hydrolysis step to the EPS extracts, while ...
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Effect of full-sib and S2-selection on a sweet corn population (Zea mays convar. saccharata)
(2024-04-26)
Sweet corn breeding goals differ from grain or silage corn. Sweet corn goals focus on marketable yield including several quality traits. This study explores the effect of a single cycle of full-sib selection and S2 selection on improving the marketable yield of an open-pollinated sweet corn population. The selected populations were subsequently compared in four environments for several plant-, yield- and quality traits relative to the original population. Analysis of variance was used to detect selection progress and ...
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Fluoropyrimidines trigger decay of hypomodified tRNA in yeast
(2024-06-10)
Therapeutic fluoropyrimidines 5-fluorouracil (5-FU) and 5-fluorocytosine (5-FC) are in long use for treatment of human cancers and severe invasive fungal infections, respectively. 5-Fluorouridine triphosphate represents a bioactive metabolite of both drugs and is incorporated into target cells’ RNA. Here we use the model fungus Saccharomyces cerevisiae to define fluorinated tRNA as a key mediator of 5-FU and 5-FC cytotoxicity when specific tRNA methylations are absent. tRNA methylation deficiency caused by loss of ...
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Influence of self-assessment and conditional metaconceptual knowledge on students' self-regulation of intuitive and scientific conceptions of evolution
(2024-02-28)
Intuitive conceptions based on cognitive biases (teleology, anthropomorphism, and essentialism) often prove helpful in everyday life while simultaneously being problematic in scientific contexts. Nonetheless, students often have intuitive conceptions of scientific topics such as evolution. As potential approaches to enable students to selfregulate their conceptions in the context of evolution, we investigated the effectiveness of two instructional approaches that are based on metacognition and selfregulated learning: ...