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Artikel (Publikationen im Open Access gefördert durch die UB)
tRNA anticodon loop modifications ensure protein homeostasis and cell morphogenesis in yeast
Abstract
Using budding yeast, we investigated a negative interaction network among genes for tRNA modifications previously implicated in anticodon-codon interaction: 5-methoxy-carbonyl-methyl-2-thio-uridine (mcm5s2U34: ELP3, URM1), pseudouridine (Ψ38/39: DEG1) and cyclic N6-threonyl-carbamoyl-adenosine (ct6A37: TCD1). In line with functional cross talk between these modifications, we find that combined removal of either ct6A37 or Ψ38/39 and mcm5U34 or s2U34 results in morphologically altered cells with synthetic growth defects. Phenotypic suppression by tRNA overexpression suggests that these defects are caused by malfunction of tRNALysUUU or tRNAGlnUUG, respectively. Indeed, mRNA translation and synthesis of the Gln-rich prion Rnq1 are severely impaired in the absence of Ψ38/39 and mcm5U34 or s2U34, and this defect can be rescued by overexpression of tRNAGlnUUG. Surprisingly, we find that combined modification defects in the anticodon loops of different tRNAs induce similar cell polarity- and nuclear segregation defects that are accompanied by increased aggregation of cellular proteins. Since conditional expression of an artificial aggregation-prone protein triggered similar cytological aberrancies, protein aggregation is likely responsible for loss of morphogenesis and cytokinesis control in mutants with inappropriate tRNA anticodon loop modifications.
Citation
in: Nucleic Acids Research. - Oxford : Oxford Univ. Press. - 2016, Vol. 44, 22, 1-14Sponsorship
Gefördert durch den Publikationsfonds der Universität KasselCollections
Publikationen (Fachgebiet Mikrobiologie)Artikel (Publikationen im Open Access gefördert durch die UB)
Citation
@article{urn:nbn:de:hebis:34-2016110951386,
author={Klassen, Roland and Ciftci, Akif and Funk, Johanna and Bruch, Alexander and Butter, Falk and Schaffrath, Raffael},
title={tRNA anticodon loop modifications ensure protein homeostasis and cell morphogenesis in yeast},
year={2016}
}
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2016-11-09T14:17:55Z 2016-11-09T14:17:55Z 2016-08-05 1362-4962 0305-1048 urn:nbn:de:hebis:34-2016110951386 http://hdl.handle.net/123456789/2016110951386 Gefördert durch den Publikationsfonds der Universität Kassel eng Urheberrechtlich geschützt https://rightsstatements.org/page/InC/1.0/ 570 tRNA anticodon loop modifications ensure protein homeostasis and cell morphogenesis in yeast Aufsatz Using budding yeast, we investigated a negative interaction network among genes for tRNA modifications previously implicated in anticodon-codon interaction: 5-methoxy-carbonyl-methyl-2-thio-uridine (mcm5s2U34: ELP3, URM1), pseudouridine (Ψ38/39: DEG1) and cyclic N6-threonyl-carbamoyl-adenosine (ct6A37: TCD1). In line with functional cross talk between these modifications, we find that combined removal of either ct6A37 or Ψ38/39 and mcm5U34 or s2U34 results in morphologically altered cells with synthetic growth defects. Phenotypic suppression by tRNA overexpression suggests that these defects are caused by malfunction of tRNALysUUU or tRNAGlnUUG, respectively. Indeed, mRNA translation and synthesis of the Gln-rich prion Rnq1 are severely impaired in the absence of Ψ38/39 and mcm5U34 or s2U34, and this defect can be rescued by overexpression of tRNAGlnUUG. Surprisingly, we find that combined modification defects in the anticodon loops of different tRNAs induce similar cell polarity- and nuclear segregation defects that are accompanied by increased aggregation of cellular proteins. Since conditional expression of an artificial aggregation-prone protein triggered similar cytological aberrancies, protein aggregation is likely responsible for loss of morphogenesis and cytokinesis control in mutants with inappropriate tRNA anticodon loop modifications. open access in: Nucleic Acids Research. - Oxford : Oxford Univ. Press. - 2016, Vol. 44, 22, 1-14 Klassen, Roland Ciftci, Akif Funk, Johanna Bruch, Alexander Butter, Falk Schaffrath, Raffael doi:10.1093/nar/gkw705
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