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Dissertation
Nachweis und Analyse BamA-assoziierter Faltungsintermediate des OmpA durch ortsgerichtete Fluoreszenzspektroskopie
(2020-05)
Die Faltung und Insertion von Außenmembranproteinen (OMPs) in die Außenmembran von Gram negativen Bakterien ist für das Leben der Zellen essentiell und erfordert den konservierten β-barrel assembly machinery (BAM) Komplex. Zahlreiche Studien befassen sich mit der Aufklärung des dahinterstehenden Faltungsmechanismus von OMPs, doch bislang ist nur sehr wenig darüber bekannt. Daher steht der Faltungsmechanismus der spontanen Faltung und Insertion von OMPs wie OmpA und der Einfluss der BAM Hauptkomponente BamA darauf, ...
Dissertation
Association of neighboring β-Strands to form the β-barrel structure of the voltage-dependent anion channel, human isoform 1 (hVDAC1) precedes membrane insertion and is largely driven by polar interactions between basic and acidic amino acid side-chains
(2020)
To date, all biophysical folding studies on β-barrel membrane proteins from outer membranes (OMPs) indicated a coupled mechanism of folding and membrane insertion. Most of these studies were performed with smaller OMPs from bacteria. Here we have investigated folding and insertion of a mitochondrial OMP, the voltage-dependent anion-selective channel (VDAC), human isoform 1 (hVDAC1). To examine whether folding and insertion are coupled for hVDAC1, the association of neighboring β-strands of the 19-stranded β-barrel ...
Dissertation
The tRNA binding protein Kti12 and its role in the regulation of Elongator complex
(2020-06-08)
[Summary] Kti12 is a conserved eukaryotic protein showing sequence homology to O-phosphoseryltRNA kinase (Pstk), which is required for the synthesis of selenocysteine tRNA. Saccharomyces cerevisiae Kti12 was originally discovered in a genetic screening for resistance against zymocin, a protein toxin produced by Kluyveromyces lactis. The cytotoxicity of zymocin lies in its anticodon nuclease activity, causing tRNA depletion and cell death in sensitive yeast cells. It recognizes and cleaves a particular wobble uridine ...
Dissertation
Investigation of the functional synergy and involvement of different tRNA modifications in translational control
(2020-07-02)
Transfer RNAs are essential adapter molecules employed in the translational process and are highly modified all over their structure. Specific modification of the four canonical ribonucleotides (A, U, C, G) is accomplished by various proteins and/or protein complexes, ranging from simple isomerisations (e.g. Ψ) and methylations (e.g. m5C) up to complex chemical structures (e.g. mcm5s2U, ct6A). The functions of tRNA modifications are linked to the specific position and may influence translation by supporting the native ...
Dissertation
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Ausprägung und Entwicklung wissenschaftlichen Denkens in der Professionalisierung von Lehr-amtsstudierenden
(2021-09)
Wissenschaftliches Denken ermöglicht Lehramtsstudierenden auf der einen Seite den Prozess na-turwissenschaftlicher Erkenntnisgewinnung zu durchdringen, zu evaluieren und problemlösend anzuwenden. Auf der anderen Seite ist es eine wesentliche Voraussetzung, um standardbezoge-nen Unterricht im Kompetenzbereich der Erkenntnisgewinnung zu konzipieren. Dabei handelt es sich jedoch nicht um intuitive und angeborene Kenntnisse und Fertigkeiten, sondern es bedarf einer qualitativen Schulung. Im Lehramtsstudium sollte darauf ...
Dissertation
Diphthamide modified eEF2: Insights into Function, Crosstalk and Disease
(2020)
Diphthamid ist eine posttranslationale Proteinmodifikation eines konservierten Histidins an dem eukaryotischen und archaealen translations-elongations Faktor 2 (eEF2 und aEF2). Obwohl die Diphthamidbiosynthese ein komplexer und gut beschriebener Ablauf ist, gibt es bisher keine detaillierte Beschreibung einer positiven zellulären Funktion der Modifikation.
Dissertation
The Importance of tRNA Modification and SSD1 Status for Protein Homeostasis and Cell Viability
(2022-03)
Chemical modifications of anticodon loop are required to support tRNA function in mRNA decoding and to prevent protein aggregation in the eukaryotic model system Saccharomyces cerevisiae. This work investigates the effects of mutation in the gene encoding the Deg1 pseudouridine synthase. It is known that the mutation of the human DEG1 orthologue PUS3 causes severe neurological and developmental defects, emphasizing the general importance of the modification. However, the exact cellular consequences of deg1/pus3mutation ...
Dissertation
Development of digital fluorescence light sheet microscopy for high-resolution optical imaging
(2021-12-15)
In modern biology investigating the morphological development of an organism is an inevitable task. Optical microscopy has been extensively used to monitor the live tissue changes in a model organism and it is typically used in conjunction with white light or fluorescence microscopy. The fluorescence microscopy occupies a significant place in biological research due to its ability to address various biological question at subcellular level of a biological sample. One of the more recent developments in fluorescence ...
Dissertation
Vergleichende phylogeographische und populationsgenetische Untersuchungen an der Pionierbaumgattung Macaranga (Euphorbiaceae) in Sundaland
(2022)
Die vorliegende Arbeit beschäftigt sich mit der Phylogeographie und Populationsgenetik der Pionierbaumgattung Macaranga in Sundaland, einer Region, die die Großen Sundainseln Sumatra, Borneo, Java und die malaiische Halbinsel umfasst. Eine Vielzahl der 300 in dieser Gattung enthaltenen Arten sind Wiederbesiedler gestörter Areale und sind daher in Sekundärwaldstrukturen besonders häufig. Im Rahmen dieser Dissertation wurden Studien in ganz Sundaland zu den sechs Arten M. tanarius (Tanarius-Artengruppe), M. gigantea, ...
Dissertation
Functional characterization of disease-associated mutations in type I cGMP-dependent protein kinase
(2022-08)
Cardiovascular diseases are the leading cause of death worldwide, and while mainly associated with behavioral risk factors, they can also be linked to genetic disorders. The cGMP-dependent protein kinase type I (PKG I) plays a pivotal role in the homeostasis of the vasculature by mediating NO/cGMP-dependent relaxation of vascular smooth muscle cells. A recently described genetic risk factor associated with PKG I results in heritable thoracic aortic aneurysms and dissections (TAAD). Although PKG isoforms Iα and Iβ ...