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Aufsatz A simple retroelement based knock-down system in Dictyostelium 

Friedrich, Michael; Meier, Doreen; Schuster, Isabelle; Nellen, Wolfgang (PLoS, 2015)
Characteristics of DIRS-1 Mediated Knock-Downs __ We have previously shown that the most abundant Dictyostelium discoideum retroelement DIRS-1 is suppressed by RNAi mechanisms. Here we provide evidence that both inverted terminal repeats have strong promoter activity and that bidirectional expression apparently generates a substrate for Dicer. A cassette containing the inverted terminal repeats and a fragment of a gene of interest was sufficient to activate the RNAi response, resulting in the generation of ~21 ...
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Aufsatz SSPIM: a beam shaping toolbox for structured selective plane illumination microscopy 

Aakhte, Mostafa; Akhlaghi, Ehsan A.; Müller, Hans-Arno J. (2018-07-03)
Selective plane illumination microscopy (SPIM) represents a preferred method in dynamic tissue imaging, because it combines high spatiotemporal resolution with low phototoxicity. The OpenSPIM system was developed to provide an accessible and flexible microscope set-up for non-specialist users. Here, we report Structured SPIM (SSPIM), which offers an open-source, user-friendly and compact toolbox for beam shaping to be applied within the OpenSPIM platform. SSPIM is able to generate digital patterns for a wide range ...
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Aufsatz Requirement of the Dynein-Adaptor Spindly for Mitotic and Post-Mitotic Functions in Drosophila 

Clemente, Giuliana D.; Hannaford, Matthew R.; Beati, Hamze; Kapp, Katja; Januschke, Jens; Griffis, Eric R.; Müller, Hans-Arno J. (2018-03-30)
Spindly was originally identified as a specific regulator of Dynein activity at the kinetochore. In early prometaphase, Spindly recruits the Dynein/Dynactin complex, promoting the establishment of stable kinetochore-microtubule interactions and progression into anaphase. While details of Spindly function in mitosis have been worked out in cultured human cells and in the C. elegans zygote, the function of Spindly within the context of an organism has not yet been addressed. Here, we present loss- and gain-of-function ...
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Aufsatz Localization and organization of protein factors involved in chromosome inheritance in Dictyostelium discoideum 

Kaller, Markus; Földesi, Balint; Nellen, Wolfgang (2007)
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Aufsatz Novel thioredoxin targets in Dictyostelium discoideum identified by two-hybrid analysis: interactions of thioredoxin with elongation factor 1 α and yeast alcohol dehydrogenase 

Brodegger, Thomas; Stockmann, Anja; Oberstraß, Jürgen; Nellen, Wolfgang; Follmann, Hartmut (2004)
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Aufsatz Binding of IRE-BP to Its Cognate RNA Sequence 

Bonin, Michael; Oberstrass, Jürgen; Vogt, Ulrike; Wassenegger, Michael; Nellen, Wolfgang (2001)
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Aufsatz A database search for double-strand containing RNAs in Dictyostelium discoideum 

Gräf, Stefan; Borisova, Branimira E.; Nellen, Wolfgang; Steger, Gerhard; Hammann, Christian (2004)

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  • Date Issued 2010 - 2018 (3) 2001 - 2009 (4)
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  • Keyword (DDC)540  Chemistry  (1)570  Life sciences; biology  (7)
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