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Loss of wobble uridine modification in tRNA anticodons interferes with TOR pathway signaling
(2014)
Previous work in yeast has suggested that modification of tRNAs, in particular uridine bases in the anticodon wobble position (U34), is linked to TOR (target of rapamycin) signaling. Hence, U34 modification mutants were found to be hypersensitive to TOR inhibition by rapamycin. To study whether this involves inappropriate TOR signaling, we examined interaction between mutations in TOR pathway genes (tip41Δ, sap190Δ, ppm1Δ, rrd1Δ) and U34 modification defects (elp3Δ, kti12Δ, urm1Δ, ncs2Δ) and found the rapamycin ...
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Microsatellites from Fosterella christophii (Bromeliaceae) by de novo transcriptome sequencing on the Pacific Biosciences RS platform
(2016)
• Premise of the study: Microsatellite markers were developed in Fosterella christophii (Bromeliaceae) to investigate the genetic diversity and population structure within the F. micrantha group, comprising F. christophii, F. micrantha, and F. villosula. • Methods and Results: Full-length cDNAs were isolated from F. christophii and sequenced on a Pacific Biosciences RS platform. A total of 1590 high-quality consensus isoforms were assembled into 971 unigenes containing 421 perfect microsatellites. Thirty primer sets ...
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Signaling of Pigment-Dispersing Factor (PDF) in the Madeira Cockroach Rhyparobia maderae
(2014)
The insect neuropeptide pigment-dispersing factor (PDF) is a functional ortholog of vasoactive intestinal polypeptide, the coupling factor of the mammalian circadian pacemaker. Despite of PDF's importance for synchronized circadian locomotor activity rhythms its signaling is not well understood. We studied PDF signaling in primary cell cultures of the accessory medulla, the circadian pacemaker of the Madeira cockroach. In Ca2+ imaging studies four types of PDF-responses were distinguished. In regularly bursting type ...
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Development of 18 Polymorphic Microsatellite Markers for Vinca minor (Apocynaceae) via 454 Pyrosequencing
(2015)
• Premise of the study: Polymorphic microsatellite markers were developed in Vinca minor (Apocynaceae) to evaluate the level of clonality, population structure, and genetic diversity of the species within its native and introduced range.
• Methods and Results: A total of 1371 microsatellites were found in 43,565 reads from 454 pyrosequencing of genomic V. minor DNA. Additional microsatellite loci were mined from publicly available cDNA sequences. After several rounds of screening, 18 primer pairs flanking di-, tri-, ...
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Actuation of an elastic membrane by the use of an electrorheological valve
(2021-01-25)
The dynamic response of a fluidic actuator is investigated on the basis of numerical simulations. With this actuator a membrane is excited to oscillate by means of an electrorheological valve. For this purpose the solid and fluid domains are coupled. Furthermore, the constitutive equation of the fluid is coupled to the electric field. The dynamic response is discussed on the example of a variable stiffness of the membrane. Actuators of this type offer the potential to delay the transition of a boundary layer.
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Efficient Solution of Distributed MIP in Control of Networked Systems
(2021-01-25)
Certain classes of optimization‐based control problems stated for networked systems involving hybrid dynamics and logical constraints can be cast into Mixed‐Integer Programming (MIP) problems. Since these belong to the complexity class NP‐hard, the motivation arises to find approximations of the optimal solution by distributed solution efficiently. For the cases that the cost functional is linear or quadratic and the constraints are linear, this paper proposes an alternative to the standard centralized schemes, by ...
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Social dynamics of values, taboos and perceived threats around sacred groves in Kurdistan, Iran
(2020-11-09)
1. Sacred groves are important for the conservation of biodiversity in the Middle East, as more formal approaches to protected areas have often failed in this global biodiversity hotspot.
2. This study aims to identify the most important social values, taboos and perceived threats around sacred groves in the Kurdistan province of Iran. We test whether values, taboos and perceived threats are lost in transmission across generations, with increased urbanisation, and with modernising livelihoods.
3. Our survey of 205 ...
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Simultaneous determination of Bingham material parameters using a ball probe concrete rheometer
(2021-01-25)
A new evaluation method allowing direct determination of the Bingham parameters from ball probe rheometers. A non‐linear relationship between the direct measured variables and the material parameters is revealed through numerical parameter studies. The method was validated on two devices of the ball probe type with different dimensions. Comparison with other methods showed good agreement. It is pointed out how the evaluation method can be transferred to devices having other dimensions by performing a limited number ...
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Damage modelling for the lifetime prediction of structural adhesive joints subjected to hygro‐thermo‐mechanical long‐term loading
(2021-01-25)
A damage model is presented for the lifetime prediction of structural adhesive joints under hygro‐thermo‐mechanical loading. The damage approach is applied through a cohesive zone model together with the concept of effective stress. It consists of a temperature‐dependent creep and hygro damage evolution due to mechanical loading and chemical aging, caused by the local water concentration. The creep damage part considers multiaxiality of the loading by an equivalent stress. The model parameters are identified by means ...
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How do consumers search for and process corporate social responsibility information on food companies’ websites?
(2019-01-03)
The importance of corporate social responsibility (CSR) communication for food companies has grown substantially ever since global action plans such as the UN Sustainable Development Goals have begun targeting topics which are highly relevant to food companies. Furthermore, various food scandals have caused consumers to question current food production methods, leading to a search for more information on ethical production methods. However, it is not known how consumers search for and process information on websites ...