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Wet sieving versus dry crushing: Soil microaggregates reveal different physical structure, bacterial diversity and organic matter composition in a clay gradient
(2020-06-18)
Soil microaggregates contain particles of different sizes, which may affect their potential to store organic carbon (OC). A variety of methods can be used to isolate microaggregates from the larger soil structures, among which wet sieving approaches are widely employed. We developed a novel dry crushing method that isolates microaggregates along failure planes due to mechanical stresses rather than hydraulic pressures and compared the mechanical stability, OC contents and microbial community composition between ...
Aufsatz
Binding of the Human 14-3-3 Isoforms to Distinct Sites in the Leucine-Rich Repeat Kinase 2
(2020-04-07)
Proteins of the 14-3-3 family are well known modulators of the leucine-rich repeat kinase 2 (LRRK2) regulating kinase activity, cellular localization, and ubiquitylation. Although binding between those proteins has been investigated, a comparative study of all human 14-3-3 isoforms interacting with LRRK2 is lacking so far. In a comprehensive approach, we quantitatively analyzed the interaction between the seven human 14-3-3 isoforms and LRRK2-derived peptides covering both, reported and putative 14-3-3 binding sites. ...
Aufsatz
Diversity of Trametes (Polyporales, Basidiomycota) in tropical Benin and description of new species Trametes parvispora
(2020-03-10)
Trametes is a globally distributed genus of white-rot polypores and well sampled in temperate and boreal areas. However, the diversity, taxonomy, and phylogenetic positions of Trametes spp. are poorly known in tropical Africa. This study aims at documenting the diversity of Trametes species in Benin (tropical Africa) and their phylogenetic positions with a focus on the T. elegans species complex. Therefore, we collected specimens of Trametes from different forest types across Benin. To infer phylogenetic relationships ...
Aufsatz
Mineralization of Phosphorylated Fish Skin Collagen/Mangosteen Scaffolds as Potential Materials for Bone Tissue Regeneration
(2021-05-13)
In this study, a potential hard tissue substitute was mimicked using collagen/mangosteen porous scaffolds. Collagen was extracted from Tilapia fish skin and mangosteen from the waste peel of the respective fruit. Sodium trimetaphosphate was used for the phosphorylation of these scaffolds to improve the nucleation sites for the mineralization process. Phosphate groups were incorporated in the collagen structure as confirmed by their attenuated total reflection Fourier transform infrared (ATR-FTIR) bands. The phosphorylation ...
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Sphaeropsis sapinea and fungal endophyte diversity in twigs of Scots pine (Pinus sylvestris) in Germany
(2020-09-03)
Sphaeropsis sapinea is the causal fungal agent of Diplodia tip blight disease of Scots pine (Pinus sylvestris) and other coniferous trees of relevance to forestry in Germany. In this study, the distribution and occurrence of S. sapinea and accompanying endophytic fungi in twigs of healthy and diseased Scots pine was investigated on a spatial and temporal scale. Sampling of 26,000 twig segments from trees in 105 temperate coniferous forest stands in Germany resulted in isolation of 33,000 endophytic fungi consisting ...
Aufsatz
An Overview of the Use of Genotyping Techniques for Assessing Genetic Diversity in Local Farm Animal Breeds
(2021-07-06)
The number of local farm animal breeds is declining worldwide. However, these breeds have different degrees of genetic diversity. Measuring genetic diversity is important for the development of conservation strategies and, therefore, various genomic analysis techniques are available. The aim of the present work was to shed light on the use of these techniques in diversity studies of local breeds. In summary, a total of 133 worldwide studies that examined genetic diversity in local cattle, sheep, goat, chicken and pig ...
Aufsatz
Genetic diversity and differentiation of Olea europaea subsp. cuspidata (Wall. & G.Don) Cif. in the Hajar Mountains of Oman
(2020-10-04)
Olea europaea subsp. cuspidata (Wall. & G. Don) Cif. is one of the six subspecies important for domestication of olive described as having valuable breeding traits. It is distributed from South Africa to the Middle East and the Mediterranean region to China mainly at mid to high altitudes with adequate precipitation. The currently rather fragmented Olea europaea subsp. cuspidata populations likely represent outposts of a formerly larger and more connected area and are hence vulnerable to additional stresses such as ...
Aufsatz
New species and a new record of Phylloporia from Benin
(2021-04-23)
Species of the wood-decay genus Phylloporia (Hymenochaetaceae, Hymenochaetales, Basidiomycota) are widely distributed in the tropics. Phylloporia species are, however, morphologically and ecologically diverse, which makes morphology-based species identification challenging. In this study, we re-examined species of Phylloporia reported from Benin (West Africa). Using an integrative approach combining morphology, ecology, and phylogenetic analyses, we describe Phylloporia beninensis sp. nov. and report Phylloporia ...
Aufsatz
Carbon use efficiency and microbial functional diversity in a temperate Luvisol and a tropical Nitisol after millet litter and N addition
(2020-07-10)
Tropical soils often contain less soil organic C (SOC) and microbial biomass C (MBC) than temperate soils and, thus, exhibit lower soil fertility. The addition of plant residues and N fertilizers can improve soil fertility, which might be reflected by microbial C use efficiency (CUE) and functional diversity. A 42-day incubation study was carried out, adding leaf litter of the C4 plant finger millet (Eleusine coracana Gaertn.) and inorganic 15N fertilizer. The aim was to investigate amendment effects on CUE and ...
Aufsatz
Induction of protein aggregation and starvation response by tRNA modification defects
(2020-08-29)
Posttranscriptional modifications of anticodon loops contribute to the decoding efficiency of tRNAs by supporting codon recognition and loop stability. Consistently, strong synthetic growth defects are observed in yeast strains simultaneously lacking distinct anticodon loop modifications. These phenotypes are accompanied by translational inefficiency of certain mRNAs and disturbed protein homeostasis resulting in accumulation of protein aggregates. Different combinations of anticodon loop modification defects were ...