Search
Now showing items 11-18 of 18
Aufsatz
Wet sieving versus dry crushing: Soil microaggregates reveal different physical structure, bacterial diversity and organic matter composition in a clay gradient
(2020-06-18)
Soil microaggregates contain particles of different sizes, which may affect their potential to store organic carbon (OC). A variety of methods can be used to isolate microaggregates from the larger soil structures, among which wet sieving approaches are widely employed. We developed a novel dry crushing method that isolates microaggregates along failure planes due to mechanical stresses rather than hydraulic pressures and compared the mechanical stability, OC contents and microbial community composition between ...
Aufsatz
Binding of the Human 14-3-3 Isoforms to Distinct Sites in the Leucine-Rich Repeat Kinase 2
(2020-04-07)
Proteins of the 14-3-3 family are well known modulators of the leucine-rich repeat kinase 2 (LRRK2) regulating kinase activity, cellular localization, and ubiquitylation. Although binding between those proteins has been investigated, a comparative study of all human 14-3-3 isoforms interacting with LRRK2 is lacking so far. In a comprehensive approach, we quantitatively analyzed the interaction between the seven human 14-3-3 isoforms and LRRK2-derived peptides covering both, reported and putative 14-3-3 binding sites. ...
Aufsatz
Morphologically similar but not closely related: the long-sporedspecies of Subulicystidium (Trechisporales, Basidiomycota)
(2020-06-06)
Species boundaries and geographic distribution of corticioid fungi (resupinate Basidiomycota) are often poorly known. Ourrecent study onSubulicystidiumshowed that species diversity in this genus is at least twice as high as previously recognized. Thisre-estimation of the species diversity was based on a study of only a part of the genus. The present study sheds light on molecularand morphological diversity of three more species. We generated 27 ITS and 24 28S nuclear ribosomal DNA sequences from 49specimens labelled ...
Aufsatz
Examining the relevance of cultural ecosystem services in forest management in Europe
(2020)
The ecosystem services framework has become one of the most important paradigms in forest planning and management as a way to link the multiple provisioning, regulating, and cultural services derived from ecosystems and their benefits to human well-being. Recently, there have been multiple efforts in emphasizing the importance of cultural ecosystem services (CES). However, the consideration of CES in management models remains a challenge. In the current context of increased demand for the joint supply of multiple ...
Aufsatz
Impact of Pus1 Pseudouridine Synthase on Specific Decoding Events in Saccharomyces cerevisiae
(2020-05-07)
Pus1-dependent pseudouridylation occurs in many tRNAs and at multiple positions, yet the functional impact of this modification is incompletely understood. We analyzed the consequences of PUS1 deletion on the essential decoding of CAG (Gln) codons by tRNAGlnCUG in yeast. Synthetic lethality was observed upon combining the modification defect with destabilized variants of tRNAGlnCUG, pointing to a severe CAG-decoding defect of the hypomodified tRNA. In addition, we demonstrated that misreading of UAG stop codons by a ...
Aufsatz
Induction of protein aggregation and starvation response by tRNA modification defects
(2020-08-29)
Posttranscriptional modifications of anticodon loops contribute to the decoding efficiency of tRNAs by supporting codon recognition and loop stability. Consistently, strong synthetic growth defects are observed in yeast strains simultaneously lacking distinct anticodon loop modifications. These phenotypes are accompanied by translational inefficiency of certain mRNAs and disturbed protein homeostasis resulting in accumulation of protein aggregates. Different combinations of anticodon loop modification defects were ...
Aufsatz
Carbon use efficiency and microbial functional diversity in a temperate Luvisol and a tropical Nitisol after millet litter and N addition
(2020-07-10)
Tropical soils often contain less soil organic C (SOC) and microbial biomass C (MBC) than temperate soils and, thus, exhibit lower soil fertility. The addition of plant residues and N fertilizers can improve soil fertility, which might be reflected by microbial C use efficiency (CUE) and functional diversity. A 42-day incubation study was carried out, adding leaf litter of the C4 plant finger millet (Eleusine coracana Gaertn.) and inorganic 15N fertilizer. The aim was to investigate amendment effects on CUE and ...
Aufsatz
Genetic diversity and differentiation of Olea europaea subsp. cuspidata (Wall. & G.Don) Cif. in the Hajar Mountains of Oman
(2020-10-04)
Olea europaea subsp. cuspidata (Wall. & G. Don) Cif. is one of the six subspecies important for domestication of olive described as having valuable breeding traits. It is distributed from South Africa to the Middle East and the Mediterranean region to China mainly at mid to high altitudes with adequate precipitation. The currently rather fragmented Olea europaea subsp. cuspidata populations likely represent outposts of a formerly larger and more connected area and are hence vulnerable to additional stresses such as ...